Keratinocyte growth factor, tumor necrosis factor-alpha and interleukin-1 beta gene expression in cultured fibroblasts and keratinocytes from burned patients

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dc.contributor.authorGragnani, Alfredo [UNIFESP]
dc.contributor.authorMüller, Bruno Rafael [UNIFESP]
dc.contributor.authorSilva, Ismael Dale Cotrim Guerreiro da [UNIFESP]
dc.contributor.authorNoronha, Samuel Marcos Ribeiro de [UNIFESP]
dc.contributor.authorFerreira, Lydia Masako [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:45:33Z
dc.date.available2015-06-14T13:45:33Z
dc.date.issued2013-08-01
dc.description.abstractPURPOSE: To evaluate the gene expression of KGF, TNF-alpha and IL-1 beta in skin fibroblasts and keratinocytes cultured from burned patients. METHODS: Three patients with large burns and three patients with small burns, as well as two controls, were included. The cell culture was initiated by the enzymatic method. After extraction and purification of mRNA, qPCR was used to assess the gene expression of KGF, TNF-alpha and IL-1 beta. RESULTS: The expression of KGF was increased on average 220-fold in large burns and 33.33-fold in small burns in fibroblasts, and 11.2-fold in large burns and 3.45-fold in small burns in keratinocytes compared to healthy patients (p<0.05). Expression of TNF-alpha was not observed. IL-1 beta is down-regulated in fibroblasts of burned patients, and much more repressed in small burns (687-fold, p<0.05). In keratinocytes, the repression of IL-1 beta expression occurs in patients with small burns (28-fold), while patients with large burns express this gene intensively (15-fold). CONCLUSIONS: The study showed a quantitative pattern in the expression of KGF gene, which is more expressed according to the size of the burn. TNF-alpha was not expressed. A qualitative pattern in the expression of IL-1 beta gene was demonstrated.en
dc.description.affiliationUNIFESP-EPM Department of Surgery
dc.description.affiliationUNIFESP-EPM
dc.description.affiliationUNIFESP-EPM Department of Gynecology
dc.description.affiliationUnifespUNIFESP, EPM, Department of Surgery
dc.description.affiliationUnifespUNIFESP-EPM
dc.description.affiliationUnifespUNIFESP, EPM Department of Gynecology
dc.description.sourceSciELO
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent551-558
dc.identifierhttp://dx.doi.org/10.1590/S0102-86502013000800001
dc.identifier.citationActa Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 8, p. 551-558, 2013.
dc.identifier.doi10.1590/S0102-86502013000800001
dc.identifier.fileS0102-86502013000800001.pdf
dc.identifier.issn0102-8650
dc.identifier.scieloS0102-86502013000800001
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/7881
dc.identifier.wosWOS:000322433100001
dc.language.isoeng
dc.publisherSociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBurnsen
dc.subjectGene Expressionen
dc.subjectFibroblastsen
dc.subjectKeratinocytesen
dc.subjectTumor Necrosis Factor-alphaen
dc.subjectFibroblast Growth Factor 7en
dc.subjectInterleukin-1en
dc.titleKeratinocyte growth factor, tumor necrosis factor-alpha and interleukin-1 beta gene expression in cultured fibroblasts and keratinocytes from burned patientsen
dc.typeinfo:eu-repo/semantics/article
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