Differential chromosome sensitivity to 5-azacytidine in Alzheimer's disease

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dc.contributor.authorPayão, Spencer Luiz Marques [UNIFESP]
dc.contributor.authorSmith, M. D.
dc.contributor.authorBertolucci, Paulo Henrique Ferreira [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-06-15T17:30:21Z
dc.date.available2018-06-15T17:30:21Z
dc.date.issued1998-09-01
dc.description.abstractBackground: The methylation process in the DNA has been considered a control mechanism of gene activity, connected with genetic imprinting. 5-Azacytidine (5-AZC) is known to be a demethylation agent. Objective: We studied the cytogenetic effect of 5-AZC in Alzheimer's disease patients and in two control groups. Methods: Peripheral lymphocyte cultures derived from 8 patients with Alzheimer's disease and 8 elderly and 8 healthy young individuals, all female, were studied. The parameters investigated were: the undercondensation of constitutive heterochromatin of chromosomes 1, 9, and 16: the number of lesions in fragile sites 1q42 and 19q13; heterochromatin association, and the total number of induced lesions. Results: Our results showed a significantly increased frequency of undercondensation of chromosomes 1, 9, and 16 in Alzheimer's disease patients when compared with elderly and young healthy groups. Conclusion: These results suggest that the demethylating action of 5-AZC could reveal differential gene activity in the Alzheimer group at the level of cellular division.en
dc.description.affiliationUNIFESP, Escola Paulista Med, Dept Morfol, Disciplina Genet, BR-04023900 Sao Paulo, Brazil
dc.description.affiliationUNIFESP, Escola Paulista Med, Dept Neurol, BR-04023900 Sao Paulo, Brazil
dc.description.affiliationUnifespUNIFESP, Escola Paulista Med, Dept Morfol, Disciplina Genet, BR-04023900 Sao Paulo, Brazil
dc.description.affiliationUnifespUNIFESP, Escola Paulista Med, Dept Neurol, BR-04023900 Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent267-271
dc.identifierhttps://doi.org/10.1159/000022023
dc.identifier.citationGerontology. Basel: Karger, v. 44, n. 5, p. 267-271, 1998.
dc.identifier.doi10.1159/000022023
dc.identifier.issn0304-324X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/43730
dc.identifier.wosWOS:000075466500003
dc.language.isoeng
dc.publisherKarger
dc.relation.ispartofGerontology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.karger.com/Services/RightsPermissions
dc.subjectAlzheimer's diseaseen
dc.subjectmethylationen
dc.subject5-azacytidineen
dc.subjecthuman chromosomesen
dc.subjectconstitutive heterochromatinen
dc.titleDifferential chromosome sensitivity to 5-azacytidine in Alzheimer's diseaseen
dc.typeinfo:eu-repo/semantics/article
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