Effect of folic acid in a modified experimental model of anorectal malformations adriamycin-induced in rats

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dc.contributor.authorFiorindo Faria, Danilo Jose [UNIFESP]
dc.contributor.authorSimoes, Manuel de Jesus [UNIFESP]
dc.contributor.authorTeixeira, Luciana Cristina [UNIFESP]
dc.contributor.authorPinheiro Faria, Angela Tucci
dc.contributor.authorSimoes Ulhoa Cintra, Alvaro Edmundo [UNIFESP]
dc.contributor.authorMartins, Jose Luiz [UNIFESP]
dc.date.accessioned2019-01-21T10:30:09Z
dc.date.available2019-01-21T10:30:09Z
dc.date.issued2016
dc.description.abstractPURPOSE: To determine the effect of a single dose of adriamycin (ADR) to induce anorectal malformations (ARMs) and determine the effect of folic acid (FA) in this model. METHODS: Ten female Wistar rats were divided randomly in two groups. Group A - ADRen
dc.description.abstractGroup B - FA+ADR. Dams from group B received daily, since two weeks before the pregnancy to the end of pregnancy, FA (50mg/kg) by gavage. Dams from both groups received ADR (6mk/kg) by intraperitoneal injection on gestational day (GD) 8. Their fetuses were harvested by cesarean section on GD21 and were examined looking for ARMs. The thickness of anal stratified squamous epithelium (ASSE) and intestinal epithelium (IE) were analyzed. p <= 0.05*. RESULTS: 81 fetuses were harvested. The number of fetusesen
dc.description.abstractnumber of ARMsen
dc.description.abstractmean (Delta%) (+/- SD) were determined to be, respectively: ADR -41[29en
dc.description.abstract65%(+/- 37%)] versus FA+ADR - 40[04en
dc.description.abstract16%(+/- 36%)] (p=0.05). AMRs were significantly lower in FA+ADR group than in ADR group (p=0.05). The thickness (mu m) of ASSE (+/- SD) and IE (+/- SD) were measured, respectively: ADR -[25.98(+/- 0.74) and 19.48(+/- 1.68)] versus FA+ADR -[24.74(+/- 0.91) and 24.80(+/- 0.81)] (p<0.005). The thickness of IE was significantly enlarged when FA was given (p<0.005). CONCLUSIONS: Single dose of adriamycin on D8 was able to induce anorectal malformations. Folic acid reduces the number and enlarged the IE of ARMs ADR-induced.en
dc.description.affiliationPostgraduate Program in Interdisciplinary Surgical Science, Universidade Federal de São Paulo (UNIFESP), Brazil
dc.description.affiliationHistology and Structural Biology Division, Department of Morphology, UNIFESP, Sao Paulo-SP, Brazil
dc.description.affiliationUniversidade São Judas Tadeu, Sao Paulo-SP, Brazil
dc.description.affiliationDepartment of Pediatric Surgery, UNIFESP, Sao Paulo-SP, Brazil
dc.description.affiliationUnifespPostgraduate Program in Interdisciplinary Surgical Science, Universidade Federal de São Paulo (UNIFESP), Brazil
dc.description.affiliationUnifespHistology and Structural Biology Division, Department of Morphology, UNIFESP, Sao Paulo-SP, Brazil
dc.description.affiliationUnifespDepartment of Pediatric Surgery, UNIFESP, Sao Paulo-SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent22-27
dc.identifierhttp://dx.doi.org/10.1590/S0102-865020160010000004
dc.identifier.citationActa Cirurgica Brasileira. Sao paulo, v. 31, n. 1, p. 22-27, 2016.
dc.identifier.doi10.1590/S0102-865020160010000004
dc.identifier.fileS0102-86502016000100022.pdf
dc.identifier.issn0102-8650
dc.identifier.scieloS0102-86502016000100022
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/49619
dc.identifier.wosWOS:000369657900004
dc.language.isoeng
dc.publisherActa cirurgica brasileira
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAnal Canalen
dc.subjectAnusen
dc.subjectImperforateen
dc.subjectDoxorubicinen
dc.subjectFolic Aciden
dc.subjectRatsNeural-Tube Defectsen
dc.subjectEmbryonic-Developmenten
dc.subjectHindgut Developmenten
dc.subjectMouse Modelen
dc.subjectSupplementationen
dc.subjectNotochorden
dc.subjectEthylenethioureaen
dc.subjectExpressionen
dc.subjectPregnancyen
dc.subjectAnomaliesen
dc.titleEffect of folic acid in a modified experimental model of anorectal malformations adriamycin-induced in ratsen
dc.typeinfo:eu-repo/semantics/article
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