Sakuranetin reverses vascular peribronchial and lung parenchyma remodeling in a murine model of chronic allergic pulmonary inflammation

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dc.contributor.authorPedroso Sakoda, Camila Pivari [UNIFESP]
dc.contributor.authorde Toledo, Alessandra Choqueta
dc.contributor.authorPerini, Adenir
dc.contributor.authorPinheiro, Nathalia Montouro
dc.contributor.authorHiyane, Meire Ioshie
dc.contributor.authorGrecco, Simone dos Santos [UNIFESP]
dc.contributor.authorLopes Calvo Tiberio, Iolanda de Fatima
dc.contributor.authorSaraiva Camara, Niels Olsen
dc.contributor.authorMartins, Milton de Arruda
dc.contributor.authorGhilardi Lago, Joao Henrique [UNIFESP]
dc.contributor.authorRighetti, Renato Fraga
dc.contributor.authorPrado, Carla Maximo [UNIFESP]
dc.date.accessioned2019-01-21T10:29:48Z
dc.date.available2019-01-21T10:29:48Z
dc.date.issued2016
dc.description.abstractBackground and purpose: Asthma is a disease of high prevalence and morbidity that generates high costs in hospitalization and treatment. Although the airway is involved in the physiopathology of asthma, there is also evidence of the importance of vascular and lung parenchyma inflammation and remodeling, which can contribute to the functional pulmonary alterations observed in asthmatic patients. Our aim was to evaluate treatment using sakuranetin, a flavone isolated from the twigs of Baccharis retusa (Asteraceae), on vascular and lung parenchyma alterations in an experimental murine model of asthma. Methods: Male BALB/c mice were subjected to a sensitization protocol with ovalbumin for 30 days and were treated with or without sakuranetin (20 mg/kg/mice) or dexamethasone (5 mg/kg/mice)en
dc.description.abstractthen, the lungs were collected for histopathological analysis. We evaluated extracellular matrix remodeling (collagen and elastic fibers), inflammation (eosinophils and NF-kB) and oxidative stress (8-isoprostane) in the pulmonary vessels and lung parenchyma. The thickness of the vascular wall was quantified, as well as the vascular endothelial growth factor (VEGF) levels. Results: We demonstrated that sakuranetin reduced the number of eosinophils and elastic fibers in both the pulmonary vessels and the lung parenchyma, probably due to a reduction of oxidative stress and of the transcription factor NF-kB and VEGF levels in the lung. In addition, it reduced the thickness of the pulmonary vascular wall. The treatment had no effect on the collagen fibers. In most of the parameters, the effect of sakuranetin was similar to the dexamethasone effect. Conclusions and implications: Sakuranetin had anti-inflammatory and antioxidant effects, preventing vascular and distal parenchyma changes in this experimental model of asthma. (C) 2016 Elsevier GmbH. All rights reserved.en
dc.description.affiliationDepartment of Bioscience, Federal University of Sao Paulo, Santos, SP, Brazil
dc.description.affiliationDepartment of Medicine, School of Medicine, Universidade de São Paulo, São Paulo, SP, Brazil
dc.description.affiliationDepartment of Bioscience Exact and Earth Science, Universidade Federal de São Paulo, Diadema, Brazil
dc.description.affiliationDepartment of Immunology, Biological Institute, Universidade de São Paulo, São Paulo, SP, Brazil
dc.description.affiliationUnifespDepartment of Bioscience, Federal University of Sao Paulo, Santos, SP, Brazil
dc.description.affiliationUnifespDepartment of Bioscience Exact and Earth Science, Universidade Federal de São Paulo, Diadema, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2010/14831-3, 2008/55359-5, 2014/25689-4]
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [304465/2012-7, 476877/2012-1]
dc.description.sponsorshipIDFAPESP: 2010/14831-3
dc.description.sponsorshipIDFAPESP: 2008/55359-5
dc.description.sponsorshipIDFAPESP: 2014/25689-4
dc.description.sponsorshipIDCNPq: 304465/2012-7
dc.description.sponsorshipIDCNPq: 476877/2012-1
dc.format.extent615-624
dc.identifierhttps://doi.org/10.1016/j.acthis.2016.07.001
dc.identifier.citationActa Histochemica. Jena, v. 118, n. 6, p. 615-624, 2016.
dc.identifier.doi10.1016/j.acthis.2016.07.001
dc.identifier.issn0065-1281
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/49405
dc.identifier.wosWOS:000383010800008
dc.language.isoeng
dc.publisherCadernos Saude Publica
dc.relation.ispartofActa Histochemica
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectBronchial Asthmaen
dc.subjectSakuranetinen
dc.subjectRemodelingen
dc.subjectOxidative Stressen
dc.subjectPeribronchial Vesselsen
dc.subjectLung ParenchymaNf-Kappa-Ben
dc.subjectAirway Inflammationen
dc.subjectOxidative Stressen
dc.subjectMast-Cellsen
dc.subjectAsthmaen
dc.subjectFlavonoidsen
dc.subjectInhibitionen
dc.subjectAsteraceaeen
dc.subjectLuteolinen
dc.subjectDiseaseen
dc.titleSakuranetin reverses vascular peribronchial and lung parenchyma remodeling in a murine model of chronic allergic pulmonary inflammationen
dc.typeinfo:eu-repo/semantics/article
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