Lower cytokine secretion ex vivo by natural killer T cells in HIV-infected individuals is associated with higher CD161 expression

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dc.contributor.authorSnyder-Cappione, Jennifer E.
dc.contributor.authorLoo, Christopher P.
dc.contributor.authorCarvalho, Karina I. [UNIFESP]
dc.contributor.authorKuylenstierna, Carlotta
dc.contributor.authorDeeks, Steven G.
dc.contributor.authorHecht, Frederick M.
dc.contributor.authorRosenberg, Michael G.
dc.contributor.authorSandberg, Johan K.
dc.contributor.authorKallas, Esper Georges [UNIFESP]
dc.contributor.authorNixon, Douglas F.
dc.contributor.institutionUniv Calif San Francisco
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionKarolinska Univ Hosp
dc.contributor.institutionSan Francisco Gen Hosp
dc.contributor.institutionAlbert Einstein Coll Med
dc.date.accessioned2016-01-24T13:58:45Z
dc.date.available2016-01-24T13:58:45Z
dc.date.issued2009-09-24
dc.description.abstractObjective: Natural killer T (NKT) cells are efficiently targeted by HIV and severely reduced in numbers in the circulation of infected individuals. the functional capacity of the remaining NKT cells in HIV-infected individuals is poorly characterized. This study measured NKT cell cytokine production directly ex vivo and compared these responses with both the disease status and NKT subset distribution of individual patients.Methods: NKT cell frequencies, subsets, and ex-vivo effector functions were measured in the peripheral blood mononuclear cells of HIV-infected patients and healthy controls by flow cytometry. We measured cytokines from NKT cells after stimulation with either a-galactosyl ceramide-loaded CD1d dimers (DimerX-alpha GalCer) or phorbol myristate acetate and ionomycin.Results: the frequencies of NKT cells secreting interferon-gamma and tumor necrosis factor-alpha were significantly lower in HIV-infected patients than healthy controls after DimerX-alpha GalCer treatment, but responses were similar after treatment with phorbol myristate acetate and ionomycin. the magnitude of the interferon-gamma response to DimerX-alpha GalCer correlated inversely with the number of years of infection. Both interferon-gamma and tumor necrosis factor-alpha production in response to DimerX-alpha GalCer correlated inversely with CD161 expression.Conclusion: the ex-vivo Th1 responses of circulating NKT cells to CD1d-glycolipid complexes are impaired in HIV-infected patients. NKT cell functions may be progressively lost over time in HIV infection, and CD161 is implicated in the regulation of NKT cell responsiveness. (C) 2009 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkinsen
dc.description.affiliationUniv Calif San Francisco, Div Expt Med, Dept Med, San Francisco, CA 94110 USA
dc.description.affiliationUniversidade Federal de São Paulo, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, São Paulo, Brazil
dc.description.affiliationKarolinska Univ Hosp, CIM, Dept Med, Karolinska Inst, Stockholm, Sweden
dc.description.affiliationSan Francisco Gen Hosp, Posit Hlth Program, San Francisco, CA 94110 USA
dc.description.affiliationAlbert Einstein Coll Med, Jacobi Med Ctr, Bronx, NY 10467 USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, EPM, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipNIAID
dc.description.sponsorshipBrazilian Program for STD and AIDS
dc.description.sponsorshipMinistry of Health
dc.description.sponsorshipSão Paulo City Health Departmen
dc.description.sponsorshipFundacao de Arnparo a Pesquisa do Estado de São Paulo
dc.description.sponsorshipJohn E. Fogarty International Center
dc.description.sponsorshipAIDS Research Institute of the AIDS Biology Program
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipBrazilian Ministry of Education
dc.description.sponsorshipIDNIAID: R37-A152731
dc.description.sponsorshipIDMinistry of Health: 914/BRA/3014 - UNESCO/Kallas
dc.description.sponsorshipIDSão Paulo City Health Departmen: 20040.168.922-7/Kallas
dc.description.sponsorshipIDFundacao de Arnparo a Pesquisa do Estado de São Paulo: 04/15856-9/Kallas
dc.description.sponsorshipIDJohn E. Fogarty International Center: D43 TW00003
dc.format.extent1965-1970
dc.identifierhttp://dx.doi.org/10.1097/QAD.0b013e32832165134
dc.identifier.citationAids. Philadelphia: Lippincott Williams & Wilkins, v. 23, n. 15, p. 1965-1970, 2009.
dc.identifier.doi10.1097/QAD.0b013e32832165134
dc.identifier.issn0269-9370
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/31819
dc.identifier.wosWOS:000270475400004
dc.language.isoeng
dc.publisherLippincott Williams & Wilkins
dc.relation.ispartofAids
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCD161en
dc.subjectHIVen
dc.subjectinterferon-gammaen
dc.subjectnatural killer Ten
dc.subjecttumor necrosis factor-alphaen
dc.subjectalpha-galactosyl ceramideen
dc.titleLower cytokine secretion ex vivo by natural killer T cells in HIV-infected individuals is associated with higher CD161 expressionen
dc.typeinfo:eu-repo/semantics/article
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