Unpredictable chronic mild stress exerts anxiogenic-like effects and activates neurons in the dorsal and caudal region and in the lateral wings of the dorsal raphe nucleus

Página do item simplificado
dc.contributor.authorLopes, Danielle Abreu [UNIFESP]
dc.contributor.authorLemes, Jessica Alves [UNIFESP]
dc.contributor.authorMelo-Thomas, Liana
dc.contributor.authorSchor, Herbert [UNIFESP]
dc.contributor.authorAndrade, José Simões de [UNIFESP]
dc.contributor.authorMachado, Carla M.
dc.contributor.authorHorta-Junior, Jose A. C.
dc.contributor.authorCéspedes, Isabel Cristina [UNIFESP]
dc.contributor.authorViana, Milena de Barros [UNIFESP]
dc.date.accessioned2019-01-21T10:29:21Z
dc.date.available2019-01-21T10:29:21Z
dc.date.issued2016
dc.description.abstractIn previous studies, we verified that exposure to unpredictable chronic mild stress (UCMS) facilitates avoidance responses in the elevated T-maze (ETM) and increased Fos-immunoreactivity in different brain structures involved in the regulation of anxiety, including the dorsal raphe (DR). Since, it has been shown that the DR is composed of distinct subpopulations of serotonergic and non-serotonergic neurons, the present study investigated the pattern of activation of these different subnuclei of the region in response to this stress protocol. Male Wistar rats were either unstressed or exposed to the UCMS procedure for two weeks and, subsequently, analyzed for Fos-immunoreactivity (Fos-ir) in serotonergic cells of the DR. To verify if the anxiogenic effects observed in the ETM could be generalized to other anxiety models, a group of animals was also tested in the light/dark transition test after UCMS exposure. Results showed that the UCMS procedure decreased the number of transitions and increased the number of stretched attend postures in the model, an anxiogenic effect. UCMS exposure also increased Fos-ir and the number of double-labeled neurons in the mid-rostral subdivision of the dorsal part of the DR and in the mid-caudal region of the lateral wings. In the caudal region of the DR there was a significant increase in the number of Fos-ir. No significant effects were found in the other DR subnuclei. These results corroborate the idea that neurons of specific subnuclei of the DR regulate anxiety responses and are differently activated by chronic stress exposure. (C) 2015 Elsevier B.V. All rights reserved.en
dc.description.affiliationDepartment of Biosciences, Federal University of São Paulo (UNIFESP), Santos, SP, Brazil
dc.description.affiliationBehavioral Neuroscience, Experimental and Physiological Psychology, Philipps-University of Marburg, Marburg, Germany
dc.description.affiliationDepartment of Anatomy, Institute of Biosciences, São Paulo State University (UNESP), Botucatu, SP, Brazil
dc.description.affiliationUnifespDepartment of Biosciences, Federal University of São Paulo (UNIFESP), Rua Silva Jardim 136, BR-11015020 Santos, SP, Brazil.
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorshipIDFAPESP: 2013/17389-8
dc.format.extent180-186
dc.identifierhttps://doi.org/10.1016/j.bbr.2015.10.006
dc.identifier.citationBehavioural Brain Research. Amsterdam, v. 297, p. 180-186, 2016.
dc.identifier.doi10.1016/j.bbr.2015.10.006
dc.identifier.issn0166-4328
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/49186
dc.identifier.wosWOS:000367107900023
dc.language.isoeng
dc.publisherBiomed Central Ltd
dc.relation.ispartofBehavioural Brain Research
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectUnpredictable Chronic Mild Stressen
dc.subjectAnxietyen
dc.subjectDorsal Rapheen
dc.subjectFos Immunoreactivityen
dc.subjectSerotoninElevated T-Mazeen
dc.subjectSerotonergic Systemsen
dc.subjectPlus-Mazeen
dc.subjectDorsomedial Hypothalamusen
dc.subjectDefensive Behaviorsen
dc.subjectAffective-Disordersen
dc.subjectExperimental-Modelen
dc.subjectRisk-Assessmenten
dc.subjectOpen-Fielden
dc.subjectIn-Vitroen
dc.titleUnpredictable chronic mild stress exerts anxiogenic-like effects and activates neurons in the dorsal and caudal region and in the lateral wings of the dorsal raphe nucleusen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
ISS - Artigos