Vitamin E prevents cell death induced by mild oxidative stress in chicken skeletal muscle cells

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dc.contributor.authorNunes, Viviane A. [UNIFESP]
dc.contributor.authorGozzo, Andrezza Justino [UNIFESP]
dc.contributor.authorCruz-Silva, Ilana [UNIFESP]
dc.contributor.authorJuliano, Maria Aparecida [UNIFESP]
dc.contributor.authorViel, Tania A. [UNIFESP]
dc.contributor.authorGodinho, Rosely O. [UNIFESP]
dc.contributor.authorMeirelles, Flavio V.
dc.contributor.authorSampaio, Misako U. [UNIFESP]
dc.contributor.authorSampaio, Claudio A M [UNIFESP]
dc.contributor.authorAraujo, Mariana S. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T12:37:57Z
dc.date.available2016-01-24T12:37:57Z
dc.date.issued2005-07-01
dc.description.abstractApoptosis and necrosis are two forms of cell death that can occur in response to various agents and oxidative damage. in addition to necrosis, apoptosis contributes to muscle fiber loss in various muscular dystrophies as well participates in the exudative diathesis in chicken, pathology caused by dietary deficiency of vitamin E and selenium, which affects muscle tissue. We have used chicken skeletal muscle cells and bovine fibroblasts to study molecular events involved in the cell death induced by oxidative stress and apoptotic agents. the effect of vitamin E on cell death induced by oxidants was also investigated. Treatment of cells with anti-Fas antibody (50 to 400 ng/mL), staurosporine (0.1 to 100 mu M) and TNF-alpha (10 and 50 ng/mL) resulted in a little loss of Trypan blue exclusion ability. Those stimuli conducted cells to apoptosis detected by an enhancement in caspase activity upon fluorogenic substrates but this activity was not fully blocked by the caspase inhibitor Z-VAD-fmk. Oxidative stress induced by menadione (10, 100 and 250 mu M) promoted a significant reduction in cell viability (10%, 20% and 35% for fibroblasts; 20%, 30% and 75% for muscle cells, respectively) and caused an increase in caspase activity and DNA fragmentation. H2O2 also promoted apoptosis verified by caspase activation and DNA fragmentation, but in higher doses induced necrosis. Vitamin E protected cells from death induced by low doses of oxidants. Although it was ineffective in reducing caspase activity in fibroblasts, this vitamin diminished the enzyme activity in muscle cells. These data suggested that oxidative stress could activate apoptotic mechanisms; however the mode of cell death will depend on the intensity and duration of the stimulus, and on the antioxidant status of the cells. (C) 2005 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biophys, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Sci, Fac Zootecnia & Engn Alimentos, Pirassununga, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biochem, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biophys, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent225-240
dc.identifierhttp://dx.doi.org/10.1016/j.cca.2005.06.001
dc.identifier.citationComparative Biochemistry and Physiology C-toxicology & Pharmacology. New York: Elsevier B.V., v. 141, n. 3, p. 225-240, 2005.
dc.identifier.doi10.1016/j.cca.2005.06.001
dc.identifier.issn1532-0456
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28370
dc.identifier.wosWOS:000231895400002
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofComparative Biochemistry and Physiology C-toxicology & Pharmacology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectapoptosisen
dc.subjectnecrosisen
dc.subjectoxidative stressen
dc.subjectvitamin Een
dc.subjectmuscle cellsen
dc.subjectfibroblastsen
dc.subjectchickenen
dc.subjectBax and Bcl-2en
dc.titleVitamin E prevents cell death induced by mild oxidative stress in chicken skeletal muscle cellsen
dc.typeinfo:eu-repo/semantics/article
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