Effects of glucose and glutamine concentrations in human dental pulp stem cells viability. An approach for cell transplantation

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dc.contributor.authorMachado, Natasha Mendonça [UNIFESP]
dc.contributor.authorDuailibi, Silvio Eduardo [UNIFESP]
dc.contributor.authorSantos, Jennifer Adriane dos
dc.contributor.authorPenna, Vanessa
dc.contributor.authorFerreira, Lydia Masako [UNIFESP]
dc.contributor.authorDuailibi, Monica Talarico [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionScience and Technology Institute
dc.date.accessioned2015-06-14T13:47:20Z
dc.date.available2015-06-14T13:47:20Z
dc.date.issued2014-10-01
dc.description.abstractPURPOSE:To evaluate microscopic behavior and viability of dental pulp stem cells under glucose and glutamine deprivation.METHODS:Human tooth tissues were minced in isolated pieces and cultured until the desired cellular proliferation for experimental phases. Cells were cultured under variations of glucose and glutamine in both serum presence and absence, and then those cells were evaluated according to number and viability by MTT assay. The confocal microscopy analyzed cytoskeleton, nucleus, and mitochondria integrity.RESULTS:A low concentration of glucose favored cellular viability and microscopic behavior; the presence of glutamine in culture medium was favorable only when associated with glucose. The cellular biological potential in culture could be preserved in serum absence if nutritional requirements are adequate.CONCLUSION:Cell microscopic behavior and viability have demonstrated better patterns on serum-free low glucose culture medium with glutamine deprivation.en
dc.description.affiliationFederal University of São Paulo Paulista School of Medicine
dc.description.affiliationScience and Technology Institute
dc.description.affiliationUNIFESP-EPM
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Department of Surgery
dc.description.affiliationUNIFESP-EPM Surgery Department
dc.description.affiliationUnifespUNIFESP, Paulista School of Medicine
dc.description.affiliationUnifespUNIFESP-EPM
dc.description.affiliationUnifespUNIFESP, Department of Surgery
dc.description.affiliationUnifespUNIFESP, EPM Surgery Department
dc.description.sourceSciELO
dc.format.extent658-666
dc.identifierhttp://dx.doi.org/10.1590/S0102-8650201400160006
dc.identifier.citationActa Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 10, p. 658-666, 2014.
dc.identifier.doi10.1590/S0102-8650201400160006
dc.identifier.fileS0102-86502014001000658.pdf
dc.identifier.issn0102-8650
dc.identifier.scieloS0102-86502014001000658
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/8627
dc.language.isoeng
dc.publisherSociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectDental Pulpen
dc.subjectCulture Mediaen
dc.subjectGlucoseen
dc.subjectGlutamineen
dc.subjectRegenerative Medicineen
dc.titleEffects of glucose and glutamine concentrations in human dental pulp stem cells viability. An approach for cell transplantationen
dc.typeinfo:eu-repo/semantics/article
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