Size and specificity of radiopharmaceuticals for sentinel lymph node detection

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dc.contributor.authorFernandez Nunez, Eutimio Gustavo
dc.contributor.authorTeodoro, Rodrigo
dc.contributor.authorWiecek, Danielle Pereira
dc.contributor.authorSilva, Natanael Gomes da
dc.contributor.authorMartinelli, Jose Roberto
dc.contributor.authorOliveira Filho, Renato Santos de [UNIFESP]
dc.contributor.institutionInst Energet & Nucl Res
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:17:12Z
dc.date.available2016-01-24T14:17:12Z
dc.date.issued2011-09-01
dc.description.abstractBackground: Biological performance of radiotracers for sentinel node detection analyzed in the light of molecular design and dimension is not widely available.Purpose: To evaluate the effect of dextran molecular size and the presence of tissue-binding units (mannose) within the model of (99m)Tc-carbonyl conjugate for sentinel lymph node detection.Material and Methods: Four dextran conjugates with and without mannose in the chemical backbone were included. All polymers were radiolabeled using the precursor [(99m)Tc(OH(2))(3)(CO)(3)](+). Radio labeling conditions targeted the best radiochemical purity and specific activity for each radiopharmaceutical, and partition coefficients were also defined. Lymphoscintigraphy and ex-vivo biodistribution in popliteal lymph node, liver and kidneys were performed in Wistar rats. the effects of molecular weight and mannose presence were assessed by a two-level factorial design.Results: Radiochemical purity was indirectly related to molecular weight and presence of mannose in the polymer structure. All products were able to detect popliteal lymph node, however, uptake was strongly influenced by use of mannose (4-fold higher). Excretion was similarly modulated by differences in molecular weight. Mannose-enhanced lymph node uptake and higher molecule size in the range under study benefitted lymphoscintigraphic performance.Conclusion: Screening of radiopharmaceuticals for lymphoscintigraphy might improve with attention to the mentioned physico-chemical features of the molecule.en
dc.description.affiliationInst Energet & Nucl Res, Radiopharm Ctr, São Paulo, Brazil
dc.description.affiliationInst Energet & Nucl Res, Ctr Mat Sci & Technol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Fac Med, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Fac Med, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipNational Commission for Nuclear Energy (CNEN, São Paulo, Brazil)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipInternational Atomic Energy Agency (IAEA, Vienna, Austria)
dc.format.extent774-778
dc.identifierhttp://dx.doi.org/10.1258/ar.2011.100506
dc.identifier.citationActa Radiologica. London: Royal Soc Medicine Press Ltd, v. 52, n. 7, p. 774-778, 2011.
dc.identifier.doi10.1258/ar.2011.100506
dc.identifier.issn0284-1851
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34037
dc.identifier.wosWOS:000295759600016
dc.language.isoeng
dc.publisherRoyal Soc Medicine Press Ltd
dc.relation.ispartofActa Radiologica
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectRadiopharmaceuticalen
dc.subjectsentinel lymph nodeen
dc.subjecttechnetiumen
dc.subjectpharmacologyen
dc.subjectmannose receptorsen
dc.titleSize and specificity of radiopharmaceuticals for sentinel lymph node detectionen
dc.typeinfo:eu-repo/semantics/article
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