Navegando por Palavras-chave "Banco de primers"

Agora exibindo 1 - 1 de 1
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Delineamento de um banco de primers para genotipagem e avaliação do estado físico do vírus do papiloma humano(HPV)em lesões escamosas intraepiteliais cervicas
    (Universidade Federal de São Paulo (UNIFESP), 2009-03-25) Pessoa, Felipe Barbosa [UNIFESP]; Lindsey, Charles Julian [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The biological diversity of human papilloma virus (HPV) reflected by the diverse genetic structure of uncountable Papovaviridae viral types and a complex biological cycle, with loss of genomic encoding regions when integrating to host cell genome, poses serious challanges to identification of viral types as to the study of gene structure and pathogenic potential relationships. Hence, we chose to produce a primer bank consisting of the genomic regions pertaining to not only the better studied viral types as HPV 16 and 18 but to as many possible others, including leser studued types as HPV 33, 35 or 52. The latter retain significant biological and clinical relevance to HPV 16. Yet, to the present diagnostic methods are a limiting factor of HPV research. Presently the most promisimng options are the genotyping kits that rely on multiplex amplification reactions with generic primers and subsequent fragment hibridizing with sequence specific DNA probes. However genotyping, as is nowerdays, may be insuficient for epidemiological, prophilatic or even medical reaearch purposes. However, the most signnificant limitation of present day genotyping is that HPV genome gene prospecting is limited to a reduced portion of the known viral types, ignoring all other HPV types irrespective of their oncogenic potential. Further more, identification of gene expression for gene structure and pathogenicity studues is not an option with the latter approach. Hence, the present proposal for HPV prevalence and host genome studies hinges on 2 approaches. One aims at producing a primer bank for identifying individual HPV genomes, gene and gene product expression for the widest range of virus types possible, while the other aims at the conception of generic primers sets that in combination with the selective primers may yield an effective system for HPV prevalence in given populations as well as the possibility of fathoming oncogenic activity in infected patients. The development of diagnostic kits with expanded sensitivity matching the diversity of the Papovaviridae family members associated to feminine genital tract oncology would be welcomed by the clinical and scientific establishment.