Navegando por Palavras-chave "Envenomation"

Agora exibindo 1 - 1 de 1
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Efeito do veneno de Lonomia obliqua em macrófagos humanos derivados da linhagem THP-1
    (Universidade Federal de São Paulo (UNIFESP), 2020-08-27) Oliveira, Douglas Souza [UNIFESP]; Tavassi, Ana Marisa Chudzinski [UNIFESP]; Universidade Federal de São Paulo
    Envenomation caused by contact with Lonomia obliqua bristles is characterized by pain, intense systemic inflammatory reaction and disturbances in the coagulation cascade which, dependent on the severity of the case, is followed by severe clinical manifestations that can lead to fatal outcome. However, the role of immune system components in these effects is still poorly understood. Therefore, the present study evaluated the direct effect of Lonomia obliqua crude bristle extract on macrophages derived from THP-1 cell line. We used protocol for monocyte differentiation into macrophage incubating the cells with Phorbol-12-myristate-13-acetate (PMA). After obtaining and characterizing these cells, we evaluated the cytotoxic effect of the venom and its ability to modulate inflammatory markers in macrophages, such as membrane proteins and a cytokine and chemokine release profile. Our results showed that the venom had no cytotoxic effects at the different concentrations used. However, at 5 μg/mL the venom was able to directly exert a potent pro-inflammatory reaction in macrophages. Characterized by the activation of the NF-kB transcription factor pathway, increasing in co-stimulating molecules expression, such as CD80 and CD83, and a high releasing of pro-inflammatory mediators such as, TNF-α, IL-1β, IL- 6, IL-8 , CXCL10 and CCL3.These results presented in this dissertation suggest that macrophages can play an important role during the inflammatory response orchestration present in envenomation caused by Lonomia obliqua caterpillars.