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- ItemSomente MetadadadosAcupuncture reduces immune-mediated pulmonary inflammatory lesions induced in rats(Karger, 2009-01-01) Katsuya, Elizabeth Marie [UNIFESP]; Pedreira de Castro, Maria Alice [UNIFESP]; Carneiro, Celia Regina Whitaker [UNIFESP]; Yamamura, Ysao [UNIFESP]; Silveira, Vera Lucia Flor [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Introduction: the aim of this report was to experimentally demonstrate the biological actions of acupuncture in an animal model of immune-mediated inflammation associated with a deposition of collagen. Materials and Methods: Male Wistar rats were sensitized by a subcutaneous implant of heat-solidified hen egg-white and divided into 4 groups: acupuncture, sham acupuncture, immobilized, and control. Acupuncture was initiated the day after sensitization and repeated twice a week for 3 weeks. the dorsal acupoints chosen were GV-14 and BL-13, the ventral acupuncture points were LU-1, CV-17, ST-36 and SP-6. the dorsal points were stimulated manually and the ventral ones by electroacupuncture. On day 14, animals were challenged through the tail vein with Sepharose (R) beads coupled with ovalbumin. One week later, animals were bled, plasma corticosterone concentrations were measured and the lungs were removed for histological evaluation. Results: Measurement of the areas of pulmonary lesion on hematoxylin-eosin stained slides showed a significant decrease (p < 0.05) in the inflammatory infiltrate in the acupuncture group, compared to the other 3 groups. Utilization of Litt and Picrosirius staining methods, in order to better visualize the infiltrate of eosinophils and the deposition of collagen, respectively, showed that both were much less intense in the acupuncture group. Corticosterone plasma levels were similar in all groups. Conclusion: Point-specific acupuncture treatment effectively reduced the inflammatory process and the deposition of collagen around ovalbumin-Sepharose beads intravenously embolized to the lungs of rats previously sensitized with the same protein that was administered subcutaneously.
- ItemSomente MetadadadosAlterações morfológicas e morfométricas da cérvice uterina determinadas pela ministração local de hialuronidase no final da prenhez da rata albina(Rattus norvegicus albinus, Rodentia, Mammalia)(Universidade Federal de São Paulo (UNIFESP), 2003) Souza, Guilherme Negrão de [UNIFESP]; Camano, Luiz [UNIFESP]Introdução: A validade de uma revisao sistematica e metanalise depende da qualidade do planejamento, realizacao e publicacao dos estudos primarios. Considerando-se a grande variacao existente entre os estudos clinicos randomizados e controlados (ECRC), e importante que se aplique um sistema de avaliacao de qualidade, para que se tenha informacoes sobre a validade do resultado encontrado e da conclusao. Objetivos: O objetivo deste estudo foi analisar se diferentes instrumentos de avaliacao de qualidade, aplicados a um grupo de ECRC, se correlacionam e, se determinam a mesma ordenacao de qualidade destes estudos. Pretendeu-se tambem, testar se a definicao de um escore minimo de qualidade tem impacto na magnitude e/ou direcao do efeito medido na analise. Desenho: Estudo observacional e analitico. Metodo: Foram identificados 38 ECRC sobre a terapeutica da hepatite cronica pelo virus B, com Interferon Alfa, nos principais bancos de dados eletronicos. Aplicou-se aos ECRC os metodos de avaliacao de qualidade de estudos clinicos: Maastricht (M), Delphi (D) e Jadad (J). Duas epidemiologistas clinicas (EC) aplicaram, aos mesmos estudos, o sistema sugerido pela Colaboracao Cochrane, o qual foi assumido como padrao ouro de comparacao. Foi realizado teste de Correlacao de Spearman para se comparar resultados definidos pelos tres instrumentos. O teste de Kappa (K) foi realizado para se avaliar a concordancia entre os revisores, na aplicacao dos instrumentos. Utilizou-se o teste de Kappa Ponderado para se comparar o ordenamento de qualidade determinado pelos instrumentos. Foi realizada metanalise, considerando como desfecho o clareamento do HBV-DNA e HbeAg. Comparou-se os resultados, quando todos os estudos foram incluidos e, quando apenas os considerados melhores o foram, examinando-se o impacto na magnitude e direcao do efeito. Resultados: Em geral, os ECRC foram considerados de regular e baixa qualidade. A concordancia avaliada pelo teste de Kappa, entre os revisores dependeu do instrumento aplicado, situando-se entre pequena e regular (D=0.12; J=0.29 e M=0.33), sendo moderada (0,53) entre as EC. A correlacao entre os instrumentos foi moderada e homogenea (DIJ=0,51; DIM=0,53 e JIM=0,52). Quando todos os artigos identificados foram incluidos na metanalise, (desfecho negativacao do HBV-DNA), obteve-se RR = 0,71; IC 95 por cento: 0,660,77 e, RR = 0,67, 95 por cento: 0,58-0,79, quando so os melhores foram incluidos. Para o desfecho clareamento do HbeAg, os resultados foram respectivamente RR = 0,85; IC 95 por cento: 0,80-0,90 e, RR = 0,85; IC 95 por cento:0,77-0,93. Conclusoes: A concordancia entre os revisores na aplicacao dos Metodos Delphi, Jadad e Maastricht, na identificacao dea(au)
- ItemAcesso aberto (Open Access)Avaliação da resposta terapêutica nas alergias oculares por meio da citologia esfoliativa(Conselho Brasileiro de Oftalmologia, 2003-10-01) Bezerra, Haroldo de Lucena [UNIFESP]; Rizzo, Luiz Vicente; Yu, Maria Cecília Zorat [UNIFESP]; Freitas, Denise de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)PURPOSE: To evaluate the therapeutic response of ocular allergies through exfoliative cytology of conjunctival scrappings. METHOD: Conjunctival exfoliative cytology was performed through a prospective study in forty six eyes of twenty-three patients with clinical diagnosis of allergic conjunctivitis. Three scrappings were performed: (1) in the pre-treatment period, (2) after use of corticosteroids (treatment A) and (3) after use of mast cell stabilizer (treatment B); in two types of smears, with and without albumin. The smears were stained with Giemsa. RESULTS: Of the twenty-three patients, five (21.7%) were female and eighteen (78.3%) male, with an average of 10.8 years. Eighteen (78.3%) presented vernal conjunctivitis and five (21.7%) atopic keratoconjunctivitis. After statistical analysis of the conjunctival cytology, the number of degenerate epithelial cells was higher in the two treatment groups in relation to the pre-treatment period. The number of neutrophils was smaller in the A treatment group than in the B treatment and pre-treatment groups. Regarding the number of eosinophils, the two treatments presented a lower percentile distribution than that of the pre-treatment period. The number of monocytes was smaller after the A treatment than the B group and the pre-treatment period. CONCLUSION: Exfoliative conjunctival cytology is an appropriate test to evaluate the therapeutic response in ocular allergies.
- ItemSomente MetadadadosBeneficial effect of annexin A1 in a model of experimental allergic conjunctivitis(Elsevier B.V., 2015-05-01) Gimenes, Alexandre D. [UNIFESP]; Andrade, Teresa Raquel M. [UNIFESP]; Mello, Claudia B.; Ramos, Lisandra [UNIFESP]; Gil, Cristiane D. [UNIFESP]; Oliani, Sonia M. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Univ Estadual PaulistaAnnexin A1 (ANXA1), a 37 kDa glucocorticoid-regulated protein, is a potent anti-inflammatory mediator effective in terminating acute inflammatory response, and its role in allergic settings has been poorly studied. the aim of this investigation was to evaluate the mechanism of action of ANXA1 in intraocular inflammation using a classical model of ovalbumin (OVA)-induced allergic conjunctivitis (AC). OVA-immunised Balb/c mice, wild-type (WT) and ANXA1-deficient (AnxA1(-/-)), were challenged with eye drops containing OVA on days 14-16 with a subset of WT animals pretreated intraperitoneally with the peptide AC(2-26) (N-terminal region of ANXA1) or dexamethasone (DEX). After 24 h of the last ocular challenge, WT mice treated with Ac2-26 and DEX had significantly reduced clinical signs of conjunctivitis (chemosis, conjunctival hyperaemia, lid oedema and tearing), plasma IgE levels, leukocyte (eosinophil and neutrophil) influx and mast cell degranulation in the conjunctiva compared to WT controls. These anti-inflammatory effects of DEX were associated with high endogenous levels of ANXA1 in the ocular tissues as detected by immunohistochemistry. Additionally, AC(2-26) administration was effective to reduce IL-2, IL-4, IL-10, IL-13, eotaxin and RANTES in the eye and lymph nodes compared to untreated WT animals. the lack of ANXA1 produced an exacerbated allergic response as detected by the density of the inflammatory cell influx to the conjunctiva and the cytokine/chemokine release. These different effects observed for Ac2-26 were correlated with diminished level of activated ERK at 24 h in the ocular tissues compared to untreated OVA group. Our findings demonstrate the protective effect of ANXA1 during the inflammatory allergic response suggesting this protein as a potential target for new ocular inflammation therapies. (C) 2015 Elsevier B.V. All rights reserved.
- ItemSomente MetadadadosEffects of Repeated Stress on Distal Airway Inflammation, Remodeling and Mechanics in an Animal Model of Chronic Airway Inflammation(Karger, 2012-01-01) Leick, Edna Aparecida; Reis, Fabiana Gomes; Honorio-Neves, Flavia Alves; Almeida-Reis, Rafael; Prado, Carla Máximo [UNIFESP]; Martins, Milton de Arruda; Tiberio, Iolanda de Fátima Lopes Calvo; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)Background/Aims: Epidemiological studies suggest that stress has an impact on asthmatic exacerbations. We evaluated if repeated stress, induced by forced swimming, modulates lung mechanics, distal airway inflammation and extracellular matrix remodeling in guinea pigs with chronic allergic inflammation. Methods: Guinea pigs were submitted to 7 ovalbumin or saline aerosols (1-5 mg/ml during 4 weeks; OVA and SAL groups). Twenty-four hours after the 4th inhalation, guinea pigs were submitted to the stress protocol 5 times a week during 2 weeks (SAL-S and OVA-S groups). Seventy-two hours after the 7th inhalation, guinea pigs were anesthetized and mechanically ventilated. Resistance and elastance of the respiratory system were obtained at baseline and after ovalbumin challenge. Lungs were removed, and inflammatory and extracellular matrix remodeling of distal airways was assessed by morphometry. Adrenals were removed and weighed. Results: the relative adrenal weight was greater in stressed guinea pigs compared to non-stressed animals (p < 0.001). Repeated stress increased the percent elastance of the respiratory system after antigen challenge and eosinophils and lymphocytes in the OVA-S compared to the OVA group (p < 0.001, p = 0.003 and p < 0.001). Neither collagen nor elastic fiber contents were modified by stress in sensitized animals. Conclusions: in this animal model, repeated stress amplified bronchoconstriction and inflammatory response in distal airways without interfering with extracellular matrix remodeling. Copyright (C) 2011 S. Karger AG, Basel
- ItemAcesso aberto (Open Access)Eosinophilic colitis in infants(Sociedade Brasileira de Pediatria, 2014-01-01) Lozinsky, Adriana Chebar [UNIFESP]; Morais, Mauro Batista de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)OBJECTIVE:To review the literature for clinical data on infants with allergic or eosinophilic colitis.DATA SOURCE:MEDLINE search of all indexes was performed using the words ''colitis or procto-colitis and eosinophilic'' or ''colitis or proctocolitis and allergic'' between 1966 and February of 2013. All articles that described patients' characteristics were selected.DATA SYNTHESIS:A total of 770 articles were identified, of which 32 met the inclusion criteria. The 32 articles included a total of 314 infants. According to the available information, 61.6% of infants were male and 78.6% were younger than 6 months. Of the 314 patients, 49.0% were fed exclusively breast milk, 44.2% received cow's milk protein, and 6.8% received soy protein. Diarrheal stools were described in 28.3% of patients. Eosinophilia was found in 43.8% (115/263) of infants. Colonic or rectal biopsy showed infiltration by eosinophils (between 5 and 25 perhigh-power field) in 89.3% (236/264) of patients. Most patients showed improvement with theremoval of the protein in cow's milk from their diet or the mother's diet. Allergy challenge tests with cow's milk protein were cited by 12 of the 32 articles (66 patients).CONCLUSIONS:Eosinophilic colitis occurs predominantly in the first six months of life and in males. Allergy to cow's milk was considered the main cause of eosinophilic colitis. Exclusion of cow'smilk from the diet of the lactating mother or from the infant's diet is generally an effective therapeutic measure.
- ItemSomente MetadadadosEosinophilic colitis in infants(Soc Brasil Pediatria, 2014-01-01) Lozinsky, Adriana Chebar [UNIFESP]; Morais, Mauro Batista de [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objective: to review the literature for clinical data on infants with allergic or eosinophilic colitis.Data source: MEDLINE search of all indexes was performed using the words colitis or proctocolitis and eosinophilic or colitis or proctocolitis and allergic between 1966 and February of 2013. All articles that described patients' characteristics were selected.Data synthesis: a total of 770 articles were identified, of which 32 met the inclusion criteria. the 32 articles included a total of 314 infants. According to the available information, 61.6% of infants were male and 78.6% were younger than 6 months. of the 314 patients, 49.0% were fed exclusively breast milk, 44.2% received cow's milk protein, and 6.8% received soy protein. Diarrheal stools were described in 28.3% of patients. Eosinophilia was found in 43.8% (115/263) of infants. Colonic or rectal biopsy showed infiltration by eosinophils (between 5 and 25 per high-power field) in 89.3% (236/264) of patients. Most patients showed improvement with the removal of the protein in cow's milk from their diet or the mother's diet. Allergy challenge tests with cow's milk protein were cited by 12 of the 32 articles (66 patients).Conclusions: eosinophilic colitis occurs predominantly in the first six months of life and in males. Allergy to cow's milk was considered the main cause of eosinophilic colitis. Exclusion of cow's milk from the diet of the lactating mother or from the infant's diet is generally an effective therapeutic measure. (C) 2013 Sociedade Brasileira de Pediatria. Published by Elsevier Editora Ltda. All rights reserved.
- ItemSomente MetadadadosLow-level laser therapy inhibits bronchoconstriction, Th2 inflammation and airway remodeling in allergic asthma(Elsevier B.V., 2014-04-01) Silva, Vanessa Ribeiro da; Marcondes, Priscila Toledo [UNIFESP]; Silva, M.; Villaverde, Antonio Balbin; Castro-Faria-Neto, Hugo Caire; Vieira, Rodolfo de Paula [UNIFESP]; Aimbire, Flavio [UNIFESP]; Oliveira, Ana Paula L. de; Nove de Julho Univ UNINOVE; Universidade Federal de São Paulo (UNIFESP); Unicastelo; Fiocruz MSLow-level laser therapy (LLLT) controls bronchial hyperresponsiveness (BHR) associated with increased RhoA expression as well as pro-inflammatory mediators associated with NF-kB in acute lung inflammation. Herein, we explore if LLLT can reduce both BHR and Th2 cytokines in allergic asthma. Mice were studied for bronchial reactivity and lung inflammation after antigen challenge. BHR was measured through dose-response curves to acetylcholine. Some animals were pretreated with a RhoA inhibitor before the antigen. LLLT (660 nm, 30 mW and 5.4 J) was applied on the skin over the right upper bronchus and two irradiation protocols were used. Reduction of BHR post LLLT coincided with lower RhoA expression in bronchial muscle as well as reduction in eosinophils and eotaxin. LLLT also diminished ICAM expression and Th2 cytokines as well as signal transducer and activator of transduction 6 (STAT6) levels in lungs from challenged mice. Our results demonstrated that LLLT reduced BHR via RhoA and lessened allergic lung inflammation via STAT6. (C) 2014 Elsevier B.V. All rights reserved.
- ItemEmbargoPrevalência de doença pulmonar obstrutiva crônica e distribuição da contagem de eosinófilos sanguíneos em pacientes com fator de risco para DPOC (tabagismo e/ou exposição à biomassa) atendidos em quatro unidades básicas de saúde de três estados brasileiros(Universidade Federal de São Paulo, 2024-04-23) Barros, Juliana Oliveira [UNIFESP]; Jardim, José Roberto de Brito [UNIFESP]; http://lattes.cnpq.br/4320654878656075; http://lattes.cnpq.br/2625355140965779Objetivos: Descrever prevalência de DPOC e distribuição da contagem de eosinófilos em pacientes com fatores de risco para DPOC, atendidos em quatro UBS de três estados brasileiros. Métodos: Estudo multicêntrico transversal em quatro UBS (Porto Alegre, Londrina, Araraquara, Botucatu). Após avaliação médica, incluímos pacientes com ≥35 anos, tabagistas e/ou expostos à biomassa que chegaram consecutivamente à UBS por qualquer motivo e que não tinham contraindicação aos procedimentos. As variáveis avaliadas foram: dados clínicos e sociodemográficos, exposição ambiental, história tabagística, mMRC, CAT, hemograma e espirometria. O diagnóstico de DPOC foi espirométrico pela relação fixa (RF) e limite inferior da normalidade (LIN). Resultados: Foram avaliados 737 pacientes consecutivos que compareceram às UBS do estudo. A prevalência de DPOC foi de 23,7% (RF) e 26,1% (LIN). O LIN diagnosticou mais pacientes com DPOC na faixa etária entre 35 e 39 anos (7,3% LIN vs 4,6% RF) e a RF diagnosticou mais pacientes com DPOC >70 anos (30,3% RF vs 24,5% LIN). Pelo menos 50% tinham DPOC grave a muito grave. Os pacientes eram bastante sintomáticos, porém a carga de sintomas era maior no grupo DPOC (p<0,01). A RF alcançou 76% de subdiagnóstico e 12% de sobrediagnóstico. A distribuição da contagem de eosinófilos no sangue foi semelhante a estudos internacionais em países desenvolvidos (228,4 ± 260,5). No grupo DPOC, ter utilizado antiparasitários nos últimos 3 meses foi associado a contagem de eosinófilos <100 (p=0,04). Não houve associação significante entre o número de eosinófilos e as exacerbações moderadas/graves nos 12 ou 24 meses prévios, porém indivíduos com contagem >300 células apresentam 20% mais exacerbações que as demais contagens. Conclusões: Um em cada quatro pacientes tinha diagnóstico de DPOC, com alto percentual de subdiagnóstico e alta carga de sintomas. A maioria apresentava doença moderada ou grave. A contagem de eosinófilos nos pacientes com/sem DPOC apresentou distribuição semelhante em todas as categorias de contagem de eosinófilos, porém pacientes com DPOC e >300 células apresentam 20% mais exacerbações.