Navegando por Palavras-chave "Estímulo hormonal"

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    Análises metabolômica e de peroxidação lipídica do líquido folicular de pacientes submetidas ao tratamento de fertilização in vitro somente com FSH e com adição de LH
    (Universidade Federal de São Paulo (UNIFESP), 2018-05-29) Costa, Livia Do Vale Teixeira da [UNIFESP]; Fraietta, Renato [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: To assess the effect of exogenous LH addition during ovarian stimulation in the metabolomic profile and lipid peroxidation levels in the follicular fluid of women undergoing IVF. Methods: A retrospective selfparing study was performed in which 36 patients were submitted to assisted reproduction treatments under different ovarian stimulation protocols. The patients were divided into two groups: FSH group, composed of 3 6 follicular fluid samples of patients that received FSH only as gonadotropin in the first treatment; and LH group, composed of 36 samples of the same patients who returned to the assisted reproduction section and received a new stimulus, in which LH was added to treatment. Results: The analysis of the clinical data showed no statistical difference in the clinical parameters of the patients when LH was added. The metabolomic analysis showed that there was a different pattern of metabolites among the same patients submitted to different stimuli, determined by the presence of 5 categories of lipids: fatty acids, sphingolipids, glycerophospholipids, prenols and sterols. Analysis of lipid peroxidation showed that there was no statistical difference between the groups. Conclusion: From the results obtained, we could observe that there is a change in the pattern of metabolites when the exogenous LH is added to the ovarian stimulus. This finding may help in determining the most efficient protocol for each patient and, later, in the determination of therapeutic targets for personalized treatments.
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    Efeito do hormônio luteinizante (LH) adicionado ao estímulo ovariano no perfil lipídico do líquido folicular de mulheres em tratamento para infertilidade
    (Universidade Federal de São Paulo (UNIFESP), 2013-07-31) Costa, Livia do Vale Teixeira da [UNIFESP]; Fraietta, Renato Fraietta [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objectives: Using Lipidomics approach to identify the follicular fluid lipid profile of women submitted to different ovarian stimulation protocols for in vitro fertilization treatments. Method: We conducted the study using 28 self-paired samples of follicular fluid from women who presented only tubal factor as a cause of female infertility. The patients were divided into (i) FSH group, consisting of 14 patients who received FSH hormone during controlled ovarian stimulation, and (ii) FSH + LH group, composed of the same 14 patients who have returned to the sector of Assisted Human Reproduction to receive a new stimulus, which corresponded to the addition of LH to the previous protocol. Lipidomics analysis was performed by MSE mass spectrometry. Possible lipids were identified by the software SimLipid 3.4. Results: Clinical data analysis showed that there are no statistically significant differences between groups. Lipids were identified in relation to their groups, so that in the FSH group we found wax monoesters, anthocyanidins and CoA fatty acyls, while in FSH + LH group we found sphingomyelin. Conclusion: Differences were found between the two groups concerning lipid profiles analyzed in follicular fluid. These findings contributed to deepen the understanding of the molecular mechanisms involved in the administration of different hormonal stimuli for patients undergoing treatments for infertility, and so to the establishment of the best protocol for each women considering the identification of potential biomarkers.