Navegando por Palavras-chave "Eucemia Mielóide Crônica"

Agora exibindo 1 - 1 de 1
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Perfil proteolítico de células de leucemia mielóide crônica
    (Universidade Federal de São Paulo (UNIFESP), 2020-07-30) Amador, Fernanda Cardoso [UNIFESP]; Juliano, Maria Aparecida [UNIFESP]; Universidade Federal de São Paulo
    Objective: Identification and differentiation of protease classes present in Chronic Myeloid Leukemia cells (K562 and Lucena 1) by studying the interference of pH and variation of enzymatic substrates on their proteolytic activities; comparison of intracellular proteases of the two cell lines by peptidomi analysis; in silico data processing for protease mapping. Methods: Lyses of the two cell lines studied and substrate variation and pH analysis were performed in fluorescence studies by substrate hydrolysis, chromatographic peptide analysis and mass spectrometry and in silico data treatment for protease mapping. Results: It was possible to identify through the tests performed the classes of proteases present in the two cell lines and differentiate them. Most proteases found in both lines are similar, corroborating that Lucena 1 cells are similar to K562 cells – both Chronic Myeloid Leukemia cell lines - containing only the difference in the development of Lucena 1 Multiple Drug Resistance. However, the presence of protease Suppressor of tumorigenicity 14 protein (EC: 3.4.21.109) only in Lucena 1 cells concludes the difference sought in this research. Conclusions: The protease in question has been described as an adjunct in the development of Multiple Drug Resistance in Chronic Myeloid Leukemia cells treated with doxorubicin chemotherapy. The identification of this proteolytic enzyme is fundamental for the development of future treatment methods, considering that the drugs for the cure of leukemia are mostly functional, having as a misfortune the tumor cells perspicacity to develop resistance to chemotherapy.