Navegando por Palavras-chave "Fatores de crescimento"

Agora exibindo 1 - 3 de 3
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Efeitos da glicina nas expressões imunohistoquimicas de PDGF, FGF e EGF em mucosite oral induzita em hamster
    (Universidade Federal de São Paulo (UNIFESP), 2017-12-08) Sá, Odara Maria de Sousa [UNIFESP]; Caran, Eliana Maria Monteiro [UNIFESP]; Alves, Maria Teresa de Seixas [UNIFESP]; Lopes, Nilza Nelly Fontana [UNIFESP]; Maria Teresa de Seixas Alves : http://lattes.cnpq.br/0357765137541523; Nilza Nelly Fontana Lopes : http://lattes.cnpq.br/8672330651019555; http://lattes.cnpq.br/1783139918188371; http://lattes.cnpq.br/3916790331038294; Universidade Federal de São Paulo (UNIFESP)
    Introduction: Oral mucositis is one of the most frequent toxic effects of chemotherapeutic and/or radiotherapeutic treatment, resulting from complex multifaceted biological events involving DNA damage, multiple signaling, and interactions between epithelial, submucosal, and connective tissue. Clinical manifestations such as pain, difficulty in swallowing and communication, and infections have a negative impact on the tolerance to treatment and the quality of life of cancer patients. Preventive measures and curative treatment of mucositis, secondary to chemotherapy and radiotherapy, are still not well established, requiring cooperative studies and new synchronic and effective therapeutic approaches. On the other hand, the nonessential amino acid glycine has anti-inflammatory, immunomodulatory and cytoprotective action, being a potential therapeutic option in mucositis. Therefore, we performed this study with the objective of evaluating the effects of glycine on the expression of collagen and growth factors, platelet (PDGF) and epidermal (EGF), in experimental models of oral mucositis, as apoptotic markers. Methods: The biological material used was the hamster jugal mucosa. The mucositis of which was induced by the protocol proposed by Sonis. A total of 40 hamsters were used, divided into two groups: group I (n= 20)- control; Group II (n= 20) supplemented with 5% intraperitoneal glycine, 0,2 ml (2,0 mg /g) diluted in herpes . On day 7 histopathological sections were performed in order to perform the immune-histochemical method, the evaluation of quantitative and qualitative collagen expression by means of picrossirius under polarized light and the growth factors: EGF and PDGF. Results: It was observed that the group supplemented with glycine higher amounts of collagen expression and predominance type of collagen I when compared to the compared to the control group (p≤0.0002). The glycine group presented lower immunoexpression (p≤0,0001) of the growth factors, EGF and PDGF in relation to the control group. The animals that expressed type I collagen fibers, independent of the experimental group, presented higher amount of collagen and less immunoexpression of the growth factors: EGF and PDGF. Conclusion: The group supplemented with glycine showed a marked healing process of the oral mucosite, demonstrated by the predominance of collagen type I and reduction of growth factors, EGF and PDGF.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Envolvimento das proteínas fosforiladas no resíduo de tirosina no hipocampo de ratos submetidos ao status epilepticus induzido por pilocarpina
    (Universidade Federal de São Paulo (UNIFESP), 2016-02-06) Castro Neto, Eduardo Ferreira de [UNIFESP]; Mazzacoratti, Maria da Graca Naffah [UNIFESP]; http://lattes.cnpq.br/3202325307358102; http://lattes.cnpq.br/8245651230799195; Universidade Federal de São Paulo (UNIFESP)
    Protein phosphorylation on tyrosine residue has been associated with different pathologies in the nervous system, including temporal lobe epilepsy. Objective: The objective of this work was to identify the phosphotyrosine proteins involved in status epilepticus (SE) onset/maintenance and those linked to SE resistance in animals submitted to pilocarpine-induced epilepsy. Method: Three groups of animals were studied: SE Group (rats with 5 h of SE, n-=5); PSS group (pilocarpine-injected animals that did not develop SE, n=5) and Control Group (saline-treated rats, n=5). The hippocampi were isolated and submitted to proarray kit, (Full Moon BioSystems, Inc.) containing specific antibodies to identify 228 phosphoproteins. Data were analyzed using ANOVA and Tukey- Kramer as post-test and p ? 0.05 was accepted. Results: One hundred twenty three out of 228 phosphoproteins presented significant altered levels among groups. From these 123 phosphoproteins, 36 attracted our attention due to their important particularities, that is, 18 presented increased phosphorylation compared to control and non-SE groups, 12 from non-SE group displayed significant differences when compared to control and SE groups and 6 presented altered levels among all groups. Conclusion: In this study we identified proteins that are probably linked to the generation of SE from those linked to the SE resistance. Knowing pathways involved in epileptogenesis may help to understand the mechanisms responsible for different vulnerabilities to long-lasting seizures.
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    O papel da proteína morfogenética óssea na reparação do tecido ósseo
    (Sociedade Brasileira de Ortopedia e Traumatologia, 2005-01-01) Santos, Antônio Aparecido Dos; Miranda, César Dário Oliveira; Alves, Maria Teresa de Seixas [UNIFESP]; Faloppa, Flávio [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    This literature review study addresses the progresses of the use of the bone morphogenetic protein (BMP) for repairing the bone tissue and its mechanisms of action in the injured tissue. The BMPs are pleiotropic molecules that are involved in the chemotaxis, mitosis and differentiation of mesenchymal cells in the bone tissue.