Navegando por Palavras-chave "Gastric neoplasms"

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    Análise da via de sinalização WNT no câncer gástrico pela imunoexpressão das proteínas E-caderina, betacatenina, APC, TCF-4 e survivina
    (Universidade Federal de São Paulo (UNIFESP), 2016-12-21) Lins, Rodrigo Rego [UNIFESP]; Waisberg, Jaques [UNIFESP]; http://lattes.cnpq.br/8316985163665041; http://lattes.cnpq.br/6992906097701452; Universidade Federal de São Paulo (UNIFESP)
    Objective: To determine the expression of E-cadherin, betacatenin, APC, TCF-4 and survivin proteins in gastric adenocarcinoma tissues, to correlate this expression with clinicopathological parameters and to verify the possible participation of the Wnt signaling pathway in gastric carcinogenesis. Method: Clinical and anatomopathological data were collected from the medical records of 71 patients with gastric adenocarcinoma submitted to gastrectomy without neoadjuvant treatment. The material obtained in the surgery was submitted to immunohistochemical analysis to evaluate the expression of E-cadherin proteins (membrane and cytoplasm), betacatenin (membrane, cytoplasm and nucleus), APC, TCF-4 and survivin (cytoplasm and nucleus). The expression rate of each protein was analyzed according to its location in the cell and related to patient clinical and pathological variables of the tumor. Results: The expression rates of the proteins were as follows: E-cadherin (3% in the membrane, 63.6% in the cytoplasm), betacatenin (29.7% in the membrane, 23.4% in the cytoplasm, 3,1% in the nucleus), APC (94.9% in the cytoplasm, 89.8% in the nucleus), TCF-4 (25.4% in the cytoplasm, 19.4% in the nucleus) and survivin (93.9% in both cytoplasm and nucleus). There was a relationship between the expression of E-cadherin in the cytoplasm with female patients (p = 0.014), membrane betacatenin with female patients (p = 0.024), betacatenin in the cytoplasm with tumors <5cm in diameter (p = 0.041 ), APC in the cytoplasm with proximal tumors (p = 0.047), TCF-4 in the nucleus with diffuse type of Lauren classification (p = 0.017) and pT4 tumors (p = 0.002) and survivin both nucleus and cytoplasm with Lauren's intestinal tumors (p = 0.05). Conclusion: The Wnt/betacatenin is not involved in gastric carcinogenesis. However, the high frequency of survivin allows us to suggest that other signaling pathways must be involved in the transformation of gastric tissue. Positive TCF-4 immunoexpression in the cell nucleus is related to T4 gastric carcinomas and Lauren?s diffuse tumors. Positive immunoexpression of survivin in the nucleus of gastric carcinoma cells is related to Lauren?s intestinal tumors.
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    Avaliação da imunoexpressão de proteínas da via de sinalização Wnt canônica e não canônica no adenocarcinoma gástrico
    (Universidade Federal de São Paulo (UNIFESP), 2017-08-31) Oliveira, Levindo Alves de [UNIFESP]; Waisberg, Jaques [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; http://lattes.cnpq.br/8007052437699905; http://lattes.cnpq.br/8316985163665041; http://lattes.cnpq.br/0021377306376898; Universidade Federal de São Paulo (UNIFESP)
    Objectives: The aim was to analyze the expression of proteins in canonical and non-canonical Wnt signaling pathways in gastric adenocarcinoma tissue. To correlate the expression of such proteins with the patients’ biodemographical characteristics and morphological and anatomopathological characteristics of the tumor. Method: The expression of proteins in Wnt signaling pathways (Wnt-5a, FZD5, GSK3B, axin, CK1, ubiquitin, D1 cyclin and C-MYC) was evaluated in 72 patients who underwent gastric adenocarcinoma surgery. Forty-five (64.3%) were male and the age average was 64.6±11.8 years. The protein level was determined by immunohistochemistry using monoclonal antibodies in gastric tissue samples in tissue microarray. Results: The expression of CK1 protein was positive in cell cytoplasm and significant in tumors without compromised surgical margins (p= 0.031). The ubiquitin protein presented strong expression in both cell nucleus and cytoplasm and was significantly more intense in both nucleus and cytoplasm of cells of moderately and well-differentiated tumors (p= 0.037) and in intestinal tumors (p = 0.031). The D1cyclin protein was expressed only in cell nucleus and was significantly more intense in male patients’ tumors (p= 0.039). The C-MYC protein was expressed in both cell cytoplasm and nucleus and the absence of its cytoplasmic expression was significantly higher in intestinal tumors (p= 0.028) and significant odds ratio (p= 0.021). The immunoexpressions of the Wnt-5a and Frizzled-5 proteins did not present significant differences (p≤0.05) regarding the epidemiological characteristics of the patients and anatomopathological aspects of the tumor. Conclusions:The strong expression of the protein of Wnt signaling pathway suggests the relevance and involvement of the canonical pathway in the occurrence of gastric adenocarcinoma. The significant absence of expression of Wnt-5a and FZD5 proteins suggests that the non-canonical pathway is not activated in gastric adenocarcinoma tissue.
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    Qualidade de vida dos pacientes com adenocarcinoma gástrico após tratamento cirúrgico com intenção curativa
    (Universidade Federal de São Paulo (UNIFESP), 2018-08-30) Pinheiro, Rodrigo Nascimento [UNIFESP]; Lopes Filho, Gaspar De Jesus [UNIFESP]; http://lattes.cnpq.br/3518607824692081; http://lattes.cnpq.br/5988211969382265; Universidade Federal de São Paulo (UNIFESP)
    Objective: To evaluate the quality of life (QoL) and identify the association among the FACT-GA and SF36 v2 scores of the QoL outcome with the sociodemographic, clinical and anatomopathological aspects of gastric adenocarcinoma patients underwent surgery with curative intent. Methods: It was made a cross-sectional study with medical charts analysis and application of questionnaires Medical Outcomes Study 36 - Item Short Form Health Survey (MOS-SF36) and Functional Assessment of Cancer Therapy – For Patients with Gastric Cancer (FACT-Ga), version 4. A total of 104 gastric adenocarcinoma patients were enrolled from three regions of Brazil. Inferential analyzes were used in the comparisons (Mann-Whitney or Kruskal-Wallis tests) and study of relationships among scores of QoL questionnaires and the variables gender, schooling, income, tumor site, type of surgery, histopathology (Lauren types), neoadjuvant and adjuvant therapies, age, postoperative time, staging, degree cell differentiation and lymphadenectomy (Spearman correlation coefficient). Results: The women presented worse quality of life (FACT-GASTRIC total score, p=0.049) and in the PWB (physical well-being; p=0.005), EWB (emotional well-being; p=0.007), GaCS (gastric cancer subscale; p=0.011), TOI (trial outcome index; p=0.030) and mental health (p=0.011). Patients with distal tumors had better quality of life scores (FACTGASTRIC total score; p = 0.018) and in the domains GaCS (p = 0.014), TOI (p = 0.020) compared to the proximal ones. Patients with cardia tumors, on the other hand, had better physical functioning scores compared to the proximal ones (p = 0.042). Patients submitted to partial gastrectomy show better total quality of life (FACT-GASTRIC total score, p = 0.011) and in the PWB (p = 0.033), GaCS (p = 0.006), TOI (p = 0.008) than total gastrectomy patients. Not using adjuvant therapy has worse scores on bodily pain than its use (p = 0.048). The higher the lymph node staging, the worse the total quality of life (total score FACT-GASTRIC, s= -0.200, p = 0.034) and the domains GaCS (s = -0.206, p = 0.037), TOI (s = -0.216; p = 0.028) and vitality (s= -0.215, p=0.029). The longer the time after treatment, the better the role-physical score (s=0.223; p=0.023).Conclusion: The type of treatment instituted, time of postoperative and socio-demographic and anatomical factors influence the QoL