Navegando por Palavras-chave "IL-1"

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    The effect of macadamia oil intake on muscular inflammation and oxidative profile kinetics after exhaustive exercise
    (Wiley, 2017) Malvestiti, Rosane; Borges, Leandro da Silva; Weimann, Eleine; da Silva Junior, Edenilson Pinto; Levada-Pires, Adriana Cristina; Dermargos, Alexandre; Lambertucci, Rafael Herling [UNIFESP]; Hatanaka, Elaine
    In this study, we evaluated the onset and resolution of inflammation in control and macadamia oil-supplemented rats after a single session of exhaustive exercise. We also analysed the antioxidant enzymes catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), and thiobarbituric acid reactive substances (TBARS). Rats were supplemented for ten days with macadamia oil (25 mg/kg), which is rich in oleic and palmitoleic acids. On the 10th day, control and macadamia oil-treated rats exercised to physical exhaustion. Plasma and muscular levels of pro-inflammatory cytokines (tumour necrosis factor alpha [TNF-], interleukin-1 beta [IL-1], IL-6, cytokine-induced neutrophil chemoattractant-3 [CINC-3], macrophage inflammatory protein-3-alpha [MIP-3]) and soluble L-selectin were measured prior to, immediately after, and 2, 24, and 48 h after exercise. Our data revealed increases in the muscle concentrations of IL-1, L-selectin, CINC-3, and MIP-3 in the group supplemented with macadamia oil compared with the concentrations in the control group. CAT also increased in the treated group, which is important because pro-inflammatory cytokines precede growth factor production and might alter the muscle repair process. We concluded that inflammation resulting from exhaustive exercise was greater in animals that received macadamia oil than in control animals. Practical applications: There are potential benefits of using fatty acids in the treatment of injured muscles, and these substances have been used in several types of injured tissues. Macadamia oil intake alters muscle healing signaling by influencing the production of reactive oxygen species and inflammatory mediators. Therefore, it is reasonable to hypothesize that the intake of immunomodulatory supplements would be beneficial to the muscular recovery of an athlete following a strenuous exercise session.
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    Fibroblastos periodontais humanos em meio de cultura suplementado com IL-1ß no modelo de força compressiva estática continua
    (Universidade Federal de São Paulo (UNIFESP), 2016-06-30) Miguel, Delaini Pires Roman [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; http://lattes.cnpq.br/1619822351741819; http://lattes.cnpq.br/7023262511430596; Universidade Federal de São Paulo (UNIFESP)
    Introduction: The mechanotransduction may be a cellular differentiation mechanism for fibroblasts that are derived from the periodontal ligament as well as local inflammation may also play an important role in the molecular response of these cells. Objective: Evaluate the viability, apoptosis, necrosis and gene expression of proinflammatory markers in a continuous static compression model using a proinflammatory cell culture. Methods: We selected 10 patients that were submitted to third molars extractions to obtain 4 mm2 of periodontal tissue of the middle third of their roots. Fibroblasts were isolated and expanded until the 6th passage and cultured in proinflammatory culture (IL- 1beta 5 ng / mL ) and after this three groups were formed according to the applied load: Control Group (CG), no charge; Experimental Group 1 (SG1), continuous static compression of 3g/6h and Experimental Group 2 (SG2) continuous static compression 4g/6h. Results: The cell viability control group had higher results both experimental groups, and was not observed differences between experimental groups. The cell apoptosis was higher in the experimental group 2 compared to the control and experimental groups 1, with no differences between them. No differences were observed between the groups in relation to cell necrosis. In gene expression not detected in the TNF-alpha expression, but IL-6 expression, this expression equal among all groups. Conclusion: Human periodontal fibroblasts in culture medium supplemented with IL-1 beta in continuous static compressive force model showed decreased cell viability, increased apoptosis and necrosis showed no changes and gene expression of IL-6 and TNF-alpha.