Navegando por Palavras-chave "In Vitro"

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    Avaliação in vitro do efeito neuroprotetor do extrato liofilizado das folhas de Eugenia uniflora L. (Pitangueira) em cultura de células SH-SY5Y
    (Universidade Federal de São Paulo (UNIFESP), 2020-11-23) Lopes, Patricia Santos Carvalhinho [UNIFESP]; Ribeiro, Alessandra Mussi [UNIFESP]; Universidade Federal de São Paulo
    The bioprospecting of natural products for the development of new therapeutic strategies has unquestionable potential mainly because the great source of natural resources from Brazilian biodiversity. PD is a progressive neurodegenerative disease characterized by the death of dopaminergic neurons and the accumulation of alpha-synuclein protein aggregates. The etiology of PD remains unknown. Evidence shows that this disease is multifactorial related to exposure to environmental factors and genetic predisposition. Pharmacological treatment focus in the relief of symptoms but not in the PD progression. In addition, long-term use of antiparkinsonian drugs contributes to adverse effects. Studies have been developed to evaluate the potential use of plant extracts for prevention and treatment of PD. In this sense, the plant Eugenia uniflora L., popularly named to pitangueira and belonging to the family Myrtaceae, native of Brazil. Previous studies showed that E. uniflora extracts have anti-inflammatory, anti-rheumatic, antipyretic, antioxidant, antifungal activities. This study aims to identify the main phytochemicals in the extract of E, uniflora (EEu), as well as to evaluate the effects of this extract on the SH-SY5Y cell culture model. Initially, extract of dry leaves obtained and submitted to mass spectrometry to identify their main compounds. In addition, EEu was evaluated in different concentrations to analyze cell viability. Preliminary results indicate the presence of five main compounds: myricetin raminosil, oleanolic acid, galoyl-HHDP-glucose, pygenic acid A and B. Moreover, we observed that the EEu in high concentrations decreased the levels of viability of SH-SY5Y cells, in contrast in low concentrations, EEu showed a proliferative and protective effects. Our results show that the EEu is not toxic in cell culture and that it can be an important neuroprotective potential. Further studies are needed to investigate the neuroprotective potential of the EEu.
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    AZT on telomerase activity and cell proliferation in HS 839.T melanoma cells
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2012-12-01) Souza Sobrinho, Celestino Prospero de [UNIFESP]; Gragnani, Alfredo [UNIFESP]; Santos, Ivan Dunshee de Abranches Oliveira [UNIFESP]; Oliveira, Andrea Fernandes; Lipay, Monica Vanucci Nunes [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    PURPOSE: To evaluate telomerase activity and proliferation of HS839.T melanoma cells, subjected to the action of AZT. METHODS: Cells were grown in triplicate, AZT at different concentrations: 50, 100 and 200μM, was added and left for 24 and 48 hours, and its effects were compared with the control group. Telomerase activity was detected by PCR and cell proliferation was evaluated by MTT. RESULTS: After 24 hours, there was no inhibition of cell proliferation or telomerase activity when compared to the control group. After 48 hours, there was a momentary decrease, suggesting that the cell lines used in this study are sensitive to AZT, but quickly recover both the enzyme activity and cell proliferation. CONCLUSION: The action of AZT on the melanoma cells studied, at the concentrations and times tested, did not inhibit telomerase activity nor affect cell proliferation.