Navegando por Palavras-chave "Líquido cefalorraquidiano"
Agora exibindo 1 - 4 de 4
Resultados por página
Opções de Ordenação
- ItemAcesso aberto (Open Access)Estudo comparativo dos níveis de neurotransmissores no líquor de pacientes com demência com corpúsculos de Lewy, demência da doença de Alzheimer e indivíduos controles(Universidade Federal de São Paulo (UNIFESP), 2017-11-30) Miraldo, Marjorie Câmara [UNIFESP]; Mazzacoratti, Maria da Graça Naffah [UNIFESP]; Oliveira, Fabricio Ferreira de [UNIFESP]; http://lattes.cnpq.br/3204275967717703; http://lattes.cnpq.br/0945751507151552; http://lattes.cnpq.br/2144732134557538; Universidade Federal de São Paulo (UNIFESP)The early and accurate diagnosis of dementia with Lewy bodies and Alzheimer’s disease dementia needs objective and quantifiable available parameters. These syndromes have many neurodegenerative characteristics in common, which can eventually difficult their differentiation based on the neuropsychological profile. It is known that changes in the metabolism of neurotransmitters are somehow involved in these dementia syndromes. Thus, this study has as main objective the comparative study of neurotransmitter levels in CSF of patients with dementia with Lewy bodies, patients with Alzheimer's disease dementia, and controls without dementia. The levels of the main monoamines were quantified analyzing the content of their metabolites (VMA, MHPG, L-DOPA, DOPAC, 5-HIAA and HVA) as well as neurotransmitter amino acids (Asp, Glu, Gln, Gly, Tau and GABA). The identification of these neurotransmitters as possible cerebrospinal fluid biomarkers, aims at a better diagnostic differentiation among patients with such dementia syndromes. Participants with dementia with Lewy bodies (n = 20) were paired with participants with Alzheimer's disease dementia (n = 20) by gender and cognitive scores (Mini- Mental State Examination and CDR), and paired with controls without dementia (n = 20) by gender and age (± 1 year). Cerebrospinal fluid monoamine metabolites and amino acids were analyzed by high performance liquid chromatography. The concentrations of VMA, 5-HIAA and HVA were lower in both type of patients (dementia with Lewy bodies and in patients with Alzheimer’s disease dementia), when compared with controls without dementia. These data suggest a reduction in the release of norepinephrine, dopamine and serotonin by the brain of patients suffering dementia. Reduced Glu levels in CSF were also found in patients with dementia with Lewy bodies, compared with control subjects, suggesting increased release and metabolization of these neurotransmitters by the brain tissue of these patients. However, despite the alterations found, none of them had difference among the three groups. Thus, this study concluded that none of these neurotransmitters could serve as a specific marker for the dementia syndromes studied.
- ItemAcesso aberto (Open Access)Fisiopatologia da cefaléia crônica diária: estudo do líquido cefalorraquidiano(Universidade Federal de São Paulo (UNIFESP), 2008-03-26) Vieira, Domingos Sávio de Souza [UNIFESP]; Peres, Mario Fernando Prieto [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Introduction: Chronic daily headaches consist of a group of headaches, among them chronic migraine, that is comorbid with depression, overuse of medication, obesity and recently, cases of idiopathic intracranial hypertension without papilloedema. Objectives: To establish idiopathic intracranial hypertension without papilloedema prevalence and glutamate and gamma-aminobutyric acid levels in cerebrospinal fluid from patients with chronic migraine compared to other groups of patients. Methods: We studied patients with chronic migraine, who underwent lumbar puncture to rule out idiopathic intracranial hypertension without papilloedema. Amino acids glutamate and gamma-aminobutyric acid levels were measured by high performance liquid chromatography in cerebrospinal fluid. Results: Six patients, among sixty, had CSF open pressure higher than 200 mm H20 without papilloedema on fundoscopy. Patients who overused triptans had glutamate levels lower than those with abuse of other analgesic types and nonoverusers. The gamma-aminobutyric acid levels in cerebrospinal fluid were lower in depressed patients when compared to patients without depression and controls. Conclusions: The study of the cerebrospinal fluid was important in patients with chronic migraine for the exclusion of idiopathic intracranial hypertension without papilloedema, opening perspectives for the understanding of the physiopathology and development of new drug therapies for migraine and its comorbidities.
- ItemAcesso aberto (Open Access)Níveis de hipocretina-1 no líquido cefalorraquidiano de ratos tratados com antidepressivos e em um modelo animal de depressão(Universidade Federal de São Paulo (UNIFESP), 2008-01-30) Pontes, Josy Carolina Covan [UNIFESP]; Tufik, Sergio [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)O papel fisiológico das hipocretinas no ciclo sono-vigília e principalmente na manutenção da vigília é bem estabelecido, assim como o seu envolvimento na fisiopatologia da narcolepsia. Pacientes narcolépticos com baixos níveis de hipocretina exibem depressão clínica e os pacientes com depressão maior apresentam diversas alterações na arquitetura do sono. Devido a essas evidências clínicas e ao possível efeito antidepressivo desses neuropeptídeos em ratos privados de sono REM, o objetivo desse estudo foi observar se há alterações nos níveis de hipocretina-1 no estado depressivo induzido num modelo animal e após o tratamento com drogas antidepressivas. Foram usados ratos da linhagem Wistar com 3 meses de idade e realizados dois experimentos: no primeiro, os animais foram tratados durante 21 dias com drogas antidepressivas (fluoxetina e imipramina) e no segundo os animais foram submetidos a um protocolo de estresse brando crônico utilizado como modelo animal de depressão e tratados com o antidepressivo tricíclico imipramina. No primeiro experimento observamos que somente os animais tratados com imipramina tiveram os níveis de hipocretina-1 aumentados. No segundo experimento, os animais submetidos ao modelo animal de depressão apresentaram níveis mais baixos de hipocretina, os quais aumentaram após tratamento com imipramina. Esses resultados sugerem que o sistema hipocretinérgico pode ser um dos fatores envolvidos na fisiopatologia da depressão e que drogas agonistas de hipocretina podem ter efeito antidepressivo.
- ItemAcesso aberto (Open Access)Validação laboratorial de um método automatizado de dosagem da atividade de adenosina desaminase em líquido pleural e em líquido cefalorraquidiano(Sociedade Brasileira de Pneumologia e Tisiologia, 2008-12-01) Feres, Márcia Cristina [UNIFESP]; Martino, Maria Cristina de; Maldijian, Suely [UNIFESP]; Batista, Fernando; Gabriel Júnior, Alexandre [UNIFESP]; Tufik, Sergio [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Laboratório da Associação Fundo de Incentivo à Psicofarmacologia/Medicina LaboratorialOBJECTIVE: The incidence of tuberculosis worldwide has emphasized the need for better assays designed to diagnose the disease, principally the extrapulmonary form. The objective of the present study was to validate the performance of an automated method for the determination of adenosine deaminase (ADA) activity in pleural fluid (PF) and cerebrospinal fluid (CSF), comparing it with a conventional method (the modified Giusti method). METHODS: In total, 134 samples were selected from among those tested in our laboratory: 94 PF samples and 40 CSF samples. The ADA activity was determined using the two methods. Inter- and intra-assay precision was determined, linear regression analysis was performed, simple concordance tests were conducted, and the means of the differences were calculated. RESULTS: The correlation coefficients for PF and CSF samples were, respectively, 0.96 and 0.95. Inter-assay precision was determined using 21 replicates at 3 different activity levels: low, medium and high. The percentage coefficient of variation (%CV) was, respectively, 5.9, 8.1 and 5.8 for PF samples, compared with 21.9, 18.6 and 13.8 for CSF samples. Intra-assay precision in %CV was 1.3 and 11.7, respectively, for PF and CSF samples. The concordance between the methods in PF and CRF samples was, respectively, 96.8% and 100%, considering the reference values for the diagnosis of TB to be 40 U/L (conventional) and 30 U/L (automated) in PF samples, versus 9 U/L (for both methods) in CSF samples. CONCLUSIONS: The results validate the use of the automated method of determining ADA activity in PF and CSF samples as an alternative to the conventional method.