Navegando por Palavras-chave "Líquido folicular"
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- ItemAcesso aberto (Open Access)Análise do perfil metabolômico do líquido folicular de pacientes com síndrome dos ovários policísticos submetidas ao tratamento de fertilização in vitro(Universidade Federal de São Paulo (UNIFESP), 2017-03-28) Cordeiro, Fernanda Bertuccez [UNIFESP]; Lo Turco, Edson Guimaraes [UNIFESP]; Andrade, Sheila Siqueira; http://lattes.cnpq.br/0735499055744493; http://lattes.cnpq.br/0251394799200508; http://lattes.cnpq.br/3076848974586113; Universidade Federal de São Paulo (UNIFESP)Objectives: Analyze the metabolomic profile of follicular fluid from patients with and without hyper response to controlled ovarian stimulation, associated or not with polycystic ovary syndrome (PCOS). Method: Follicular fluid samples from 81 patients were obtained from patients undergoing in vitro fertilization, considering three groups: control group (n = 22); hyper responders (HR, n = 44) and PCOS (n = 15). The samples were prepared according to the respective metabolomic approaches, which included liquid-chromatography mass spectrometry (LC-MS) and Paper Spray ionization mass spectrometry (PS-MS). Results: Clinical data showed that the groups differ based on age, FSH, FSH/LH rate, number of punctured follicles, oocyte recovery rate, number of oocytes, oocytes in metaphase II and I, inseminated and fertilized oocytes, number of embryos at days 2 and 3 of cultive and on the embryo quality. The LC-MS analysis showed 14 metabolites of higher abundance, which were attributed as fatty acids, diacylglycerol, triacylglycerol, ceramide, phosphatidylcholine and sphingomyelin. The PCOS group presented one compound of higher abundance that was not attributed. The PS-MS demonstrated 9 metabolites more abundant for the control group that were attributed to fatty acids, monoacylglycerol, lyso-phosphoglycerol, lyso-sphingomyelin and sphingosine-1-phosphate. Four metabolites were of higher abundance for the PCOS group, for which the attribution was not possible. The HR group showed high abundance of 2 metabolites and one was attributed as Lyso-phosphoinositol-phosphate. Conclusion: The follicular fluid metabolomic analysis for patients with PCOS and hyper response to gonadotropins demonstrated differences among the groups that could be associated with oocyte quality and consequent embryo quality and development. In addition, the attribution of metabolites indicated that the study of these compounds may assist on elucidation of pathophysiological mechanism related to PCOS and the risk of hyper response to controlled ovarian stimulation.
- ItemSomente MetadadadosEfeito do hormônio luteinizante (LH) adicionado ao estímulo ovariano no perfil lipídico do líquido folicular de mulheres em tratamento para infertilidade(Universidade Federal de São Paulo (UNIFESP), 2013-07-31) Costa, Livia do Vale Teixeira da [UNIFESP]; Fraietta, Renato Fraietta [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objectives: Using Lipidomics approach to identify the follicular fluid lipid profile of women submitted to different ovarian stimulation protocols for in vitro fertilization treatments. Method: We conducted the study using 28 self-paired samples of follicular fluid from women who presented only tubal factor as a cause of female infertility. The patients were divided into (i) FSH group, consisting of 14 patients who received FSH hormone during controlled ovarian stimulation, and (ii) FSH + LH group, composed of the same 14 patients who have returned to the sector of Assisted Human Reproduction to receive a new stimulus, which corresponded to the addition of LH to the previous protocol. Lipidomics analysis was performed by MSE mass spectrometry. Possible lipids were identified by the software SimLipid 3.4. Results: Clinical data analysis showed that there are no statistically significant differences between groups. Lipids were identified in relation to their groups, so that in the FSH group we found wax monoesters, anthocyanidins and CoA fatty acyls, while in FSH + LH group we found sphingomyelin. Conclusion: Differences were found between the two groups concerning lipid profiles analyzed in follicular fluid. These findings contributed to deepen the understanding of the molecular mechanisms involved in the administration of different hormonal stimuli for patients undergoing treatments for infertility, and so to the establishment of the best protocol for each women considering the identification of potential biomarkers.
- ItemAcesso aberto (Open Access)Níveis de antioxidantes enzimáticos e produtos da peroxidação lipídica em líquido folicular de mulheres submetidas à estimulação ovariana controlada(Universidade Federal de São Paulo (UNIFESP), 2009-03-25) Victorino, Amanda Begatti [UNIFESP]; Bertolla, Ricardo Pimenta [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objective: to verify the relation between thiobarbituric acid reactive substances concentrations, glutathione peroxidase and catalase activities, and occurrence of pregnancy, hormonal stimulation protocol utilized, and presence or not of a female infertility factor. Method: a prospective case-control study was carried with follicular fluid from 146 patients submitted to the Assisted Reproduction program at the Sao Paulo Federal University. Only patients submitted to in vitro fertilization (FIV) through intracytoplasmic sperm injection (ICSI) were included. The experimental groups were subdivided according to presence or absence of pregnancy, hormonal stimulation protocol used (FSH or FSH and hMG) and presence or absence of at least one female infertility factor. An aliquot of follicular fluid was collected after retrieval of the oocyte for analysis of thiobarbituric acid reactive substances concentrations, and glutathione peroxidase and catalase activities. All these analyses were carried out using a spectrophotometer. Groups were compared using Student’s T-test. A logistic regression model were calculated using embryo quality on day 3, presence or not of a female infertility factor, thiobarbituric acid reactive substances levels, and glutathione peroxidase (GPX) and catalase activities as independent variables, occurrence of pregnancy as a binary dependent variable. Results: Patients who did not achieve pregnancy presented higher glutathione peroxidase activity levels, when compared with patients who achieved pregnancy (p=0,04). In the other groups, no differences were observed. Logistic regression produced a model which best predicted pregnancy including presence of female infertility factor, embryo quality on day 3 and GPX activity (73.7% total predictive value, p=0.00001). Odds-ratio for GPX activity was 0.98, indicating that increasing values of GPX activity decreased the odds of occurrence of pregnancy. Conclusion: Based on our results, we may conclude that (i) higher GPX activity is associated to negative outcomes in ICSI cycles, (ii) different ovarian stimulation protocols does not alter concentrations of TBARS and activity of antioxidant enzymes, and (iii) the presence of at least one female infertility factor does not alter TBARS levels or antioxidant activity.
- ItemAcesso aberto (Open Access)Perfil metabolômico comparativo do líquido folicular de mulheres que alcançaram a gestação em tratamentos de fertilização in vitro(Universidade Federal de São Paulo (UNIFESP), 2017-10-17) Montani, Daniela Antunes [UNIFESP]; Fraietta, Renato [UNIFESP]; http://lattes.cnpq.br/1545035937368744; http://lattes.cnpq.br/3170825336610377; Universidade Federal de São Paulo (UNIFESP)Objetivos: este estudo comparou o perfil metabolômico do líquido folicular de mulheres submetidas à injeção intracitoplasmática de espermatozoide, com o objetivo de verificar o potencial de gravidez e uma assinatura metabolômica dos resultados de gravidez em tratamentos de infertilidade. Métodos: Foi coletado o líquido folicular de 84 pacientes submetidas a estimulação ovariana controlada. As amostras foram divididas em dois grupos, dependendo do resultado beta hCG medido 14 ou 12 dias após a transferência do embrião: beta hCG positivo (n = 32) e beta hCG negativo (n = 52). Para a análise metabolômica foi realizado o protocolo de extração de Bligh & Dyer seguido de espectrometria de massa com ionização por eletropray em faixa de massa de 100-1200 m / z. Os dados dos espectros foram adquiridos em modo positivo e negativo. Os dados de metabolômica foram analisados por estatísticas multivariadas (PCA e PLS-DA) no software MetaboAnalyst 3.0. A PLS-DA foi utilizada para a seleção de biomarcadores. A curva de Característica Operacional do Receptor foi construída para os biomarcadores propostos como um conjunto e individualmente. A identificação dos biomarcadores selecionados foi feita no banco de dados on-line METLIN. A análise de enriquecimento funcional foi realizada no software CytoScape 3.5.1. Resultados: Ionização em modo positivo: foram selecionados seis íons como potenciais biomarcadores para o grupo beta hCG positivo, com valor preditivo de 99,1% e um íon foi selecionado para o grupo beta hCG negativo com valor preditivo de 96,7%. Na ionização em modo negativo, seis íons foram selecionados como biomarcadores característicos do grupo beta hCG negativo com valor preditivo de 83,5%. Ácido fosfatídico, fosfatidilserina, fosfatidiletanolamina e ceramida foram os metabólitos atribuídos ao grupo de mulheres que engravidaram, enquanto, Monoacilglicerol, diacilglicerol, fosfatidilinositol, glicuronido de ácido cólico, androstano e precursores de estradiol foram atribuídos aos íons hiperrepresentados no grupo beta hCG negativo. Na análise funcional foram observadas, regulação do processo catabólico das proteínas lisossomais e a sinalização canônica de Wnt, como vias associadas ao maior potencial de gravidez. Enquanto que, a remodelação de lipoproteínas de alta densidade (HDL) e a via da P450 hidroxilase estão associadas ao menor potencial de gravidez. Conclusão: A abordagem metabolômica do LF pode ser uma boa ferramenta para indicar o potencial de gravidez em mulheres submetidas a ICSI permitindo, desta forma, identificar com alto poder de predição as pacientes que irão se beneficiar de tratamentos de alta complexidade.