Navegando por Palavras-chave "Neuroprotective"
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- ItemAcesso aberto (Open Access)Antiepileptic-like effect of the Lippia grata extract in mice(Universidade Federal de São Paulo, 2017-12) Macedo, Amanda Maria de [UNIFESP]; Ribeiro, Alessandra Mussi [UNIFESP]; http://lattes.cnpq.br/7373640456805525; http://lattes.cnpq.br/1274034001321703; Universidade Federal de São Paulo (UNIFESP)Ethnopharmacological relevance: Brazil is responsible for managing the world's largest biodiversity asset. This rich fauna and flora can be an important source of biologically active substances with potential therapeutic use. For example, in the discovery of model drugs for the development of new drugs for the treatment of brain diseases, such as epilepsy. In this context, Lippia genus belonging to the family Verbenaceae contains compounds of different chemical classes, including terpenes, which have showed therapeutic effects. Among the pharmacological activities reported include antioxidant, anti-inflammatory, anesthetic, analgesic, anxiolytic/sedative and antihyperalgesic in rodent models. Nevertheless, few studies have been conducted to investigate possible in vivo biological effects of Lippia grata extract. Aim of the study: This study investigated a possible neuroprotective effect of the L. grata extract (LIP) on mice submitted to the model of seizures induced by pilocarpine. In addition, the behavior of the animals following acute and chronic treatment with the extract was evaluated. Results: Our main results showed that treatment with the extract (10 mg/kg) prior to the administration of pilocarpine increased latency to death and seizures-death. In short, the complexed extract L. grata can be a source of substances with anticonvulsant potential. Conclusion: Thus, further work will deal with the isolation of the active components responsible by neuroprotective effect as well as clarify their mechanism of action.
- ItemSomente MetadadadosAvaliação in vitro do efeito neuroprotetor do extrato liofilizado das folhas de Eugenia uniflora L. (Pitangueira) em cultura de células SH-SY5Y(Universidade Federal de São Paulo (UNIFESP), 2020-11-23) Lopes, Patricia Santos Carvalhinho [UNIFESP]; Ribeiro, Alessandra Mussi [UNIFESP]; Universidade Federal de São PauloThe bioprospecting of natural products for the development of new therapeutic strategies has unquestionable potential mainly because the great source of natural resources from Brazilian biodiversity. PD is a progressive neurodegenerative disease characterized by the death of dopaminergic neurons and the accumulation of alpha-synuclein protein aggregates. The etiology of PD remains unknown. Evidence shows that this disease is multifactorial related to exposure to environmental factors and genetic predisposition. Pharmacological treatment focus in the relief of symptoms but not in the PD progression. In addition, long-term use of antiparkinsonian drugs contributes to adverse effects. Studies have been developed to evaluate the potential use of plant extracts for prevention and treatment of PD. In this sense, the plant Eugenia uniflora L., popularly named to pitangueira and belonging to the family Myrtaceae, native of Brazil. Previous studies showed that E. uniflora extracts have anti-inflammatory, anti-rheumatic, antipyretic, antioxidant, antifungal activities. This study aims to identify the main phytochemicals in the extract of E, uniflora (EEu), as well as to evaluate the effects of this extract on the SH-SY5Y cell culture model. Initially, extract of dry leaves obtained and submitted to mass spectrometry to identify their main compounds. In addition, EEu was evaluated in different concentrations to analyze cell viability. Preliminary results indicate the presence of five main compounds: myricetin raminosil, oleanolic acid, galoyl-HHDP-glucose, pygenic acid A and B. Moreover, we observed that the EEu in high concentrations decreased the levels of viability of SH-SY5Y cells, in contrast in low concentrations, EEu showed a proliferative and protective effects. Our results show that the EEu is not toxic in cell culture and that it can be an important neuroprotective potential. Further studies are needed to investigate the neuroprotective potential of the EEu.