Navegando por Palavras-chave "Oligofrutose"

Agora exibindo 1 - 2 de 2
  • Imagem de Miniatura
    Item
    Acesso aberto (Open Access)
    Efeito da suplementação de ácidos graxos trans ou oligofrutose, durante a gestação e lactação, nas mães e prole com 90 dias de vida sobre o metabolismo e parâmetros inflamatórios
    (Universidade Federal de São Paulo (UNIFESP), 2016-04-30) Oliveira, Juliana Lopez de [UNIFESP]; Nascimento, Claudia Maria da Penha Oller do [UNIFESP]; Pisani, Luciana Pellegrini [UNIFESP]; Oyama, Lila Missae [UNIFESP]; http://lattes.cnpq.br/3983527783636073; http://lattes.cnpq.br/7125541171554727; http://lattes.cnpq.br/9974551337615485; http://lattes.cnpq.br/3558119106282470; Universidade Federal de São Paulo (UNIFESP)
    Introduction. Adverse effects of the consumption of trans fatty acids (TFA) have been widely studied by the scientific community in the last. Previously, our group found that the intake of trans fatty acids (TFA) during pregnancy and lactation promotes a pro-inflammatory state in the offspring at twenty-one and ninety days old. Moreover, literature data indicates that the prebiotic intake may alter the intestinal environment, presenting antiinflammatory properties. To evaluate the effect of a diet supplemented with OF (10%) or a diet rich in TFA (7%) during gestation and lactation on metabolic parameters and inflammatory lactating rats and ninety days old offspring, concerning endotoxemia, metabolic and inflammatory parameters. In addition, to assess whether the diet supplemented with OF (10%) modifies these parameters in the offspring, ninety days old offspring, of mothers who received diet containing TFA during pregnancy and lactation. Methods. On the first day of pregnancy, the rats were divided into three groups: control diet (C) control diet supplemented with 10% oligofructose (OF) and diet enriched with hydrogenated fat rich in TFA (T). The diets were maintained during pregnancy and lactation. In the twenty-first day of lactation animals were weaned and mothers euthanized. The pups were kept in individual cages within ninety days of life and than euthanazied. The blood, bowel, retroperitoneal adipose tissue (RET), parametrial fat mothers and epididymal of puppies, liver, soleus muscle tissues and EDL were collected. The serum was used for measuring lipopolysaccharide (LPS), free fatty acids (FFA), TNF- ?, hormones, insulin, ghrelin, adiponectin and NPY as well as cytokines by specific kits. The liver, and soleus and EDL RET muscle tissue were used for determination of cytokine content by specific kits. The liver and soleus muscle tissue were placed in a specific buffer to perform Western blotting. Genomic DNA Bacteria present in the colon feces was determined by real time PCR. Results. Supplementation with 10% of oligofructose during pregnancy and lactation reduced body weight gain, maternal dietary intake and serum adiponectin levels. In ninety days old offspring, maternal supplementation of oligofructose (10%) promoted increased IL-6 content and protein expression of IL-6R, and reduced protein expression of ADIPO-R1 in the soleus muscle. Maternal intake of TFA increased Lee index and the protein expression of pp65- NFkB in offspring with 90 days of life. Supplementation of offspring with
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Efeito da suplementação de oligofrutose no estado pró-inflamatório da prole com 21 dias de vida, de mães tratadas com ácidos graxos trans, durante a gestação e lactação
    (Universidade Federal de São Paulo (UNIFESP), 2014-03-26) Mennitti, Lais Vales [UNIFESP]; Pisani, Luciana Pellegrini [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Background Previously, we showed that the intake of trans fatty acids during pregnancy and lactation triggers a pro-inflammatory status in the offspring. On the other hand, prebiotics can alter the intestinal environment, reducing serum lipopolysaccharides (LPS) concentrations. This study evaluated the effect of the oligofructose 10% diet supplementation in the presence or absence of hydrogenated vegetable fat during pregnancy and lactation on the development, endotoxemia and bacterial composition of 21-d-old offspring. Methods On the first day of pregnancy rats were divided into four groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat, rich in TFA (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF). Diets were maintained during pregnancy and lactation. At birth, 7th, 14th and 21th, pups were weighed and length was measured. Serum concentrations of LPS and free fatty acids (FFA) were performed by specific kits. Bacterial DNA present in faeces was determined by real-time PCR. Data were expressed as mean ± standard error of the mean and the statistical analysis was realized by ANOVA two-way and ANOVA for repeated measures. p<0.05 was considered significant. Results We observed that the oligofructose (10%) supplementation during pregnancy and lactation reduced body weight, body weight gain, length and serum FFA in the CF and TF group compared to C and T group respectively, of the 21-day-old offspring, accompanied by an increase in serum LPS and genomic DNA levels of lactobacillus spp. on faeces of the CF group in relation to C group. Conclusion In conclusion, dam’s diet supplementation with 10% of oligofructose during pregnancy and lactation, independent of addition with hydrogenated vegetable fat, harms the offspring development, alters the bacterial composition and increases the serum concentrations of lipopolysaccharides in 21d-old pups.