Navegando por Palavras-chave "Papillomavirus infections"
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- ItemAcesso aberto (Open Access)Associação entre o papilomavírus humano de baixo e alto risco oncogênico e as lesões intraepiteliais cervicais(Universidade Federal de São Paulo (UNIFESP), 2015-06-24) Fernandes Junior, Joao Antao [UNIFESP]; Alonso, Luis Garcia [UNIFESP]; Silva, Maria Antonieta Longo Galvão da [UNIFESP]; http://lattes.cnpq.br/7276081616825195; http://lattes.cnpq.br/2101345650666445; http://lattes.cnpq.br/8915033058906887; Universidade Federal de São Paulo (UNIFESP)Objetivos: Correlacionar a detecção do Papilomavírus humano, pelo método de Captura Híbrida, com as Lesões Intraepiteliais Cervicais. Definir os grupos encontrados (tipos de HPV de baixo e alto risco) e as respectivas cargas virais, em pacientes positivos para HPV. Avaliar a persistência viral nas pacientes diagnosticadas com Lesões Intraepiteliais Cervicais. Métodos: Trata-se de um estudo observacional, descritivo e analítico, no qual foram inseridos em um banco de dados os resultados dos exames de Citologia e CH II de 112 pacientes provenientes da rotina do laboratório de Citologia e Captura Híbrida da Associação Fundo de Incentivo a Pesquisa (AFIP), no período de janeiro de 2011 a dezembro de 2012. Tais exames já foram realizados e emitidos os respectivos laudos. Foram incluídos os dados diagnósticos das pacientes que preencheram os seguintes critérios: Citologias positivas para lesões préneoplásicas - LSIL e HSIL, com diagnóstico molecular concomitante; e exames moleculares como seguimento no período de 24 meses. Resultados: 95% das LICs apresentam infecção por HPV, sendo 95% para LSIL, e 100% para HSIL. Há a presença de vírus de alto risco e de vírus de baixo e alto risco associados em aproximadamente 93% dos casos de LIC, sendo 92,2% para LSIL e 100% para HSIL. Foram encontradas cargas virais altas e muito altas na presença de vírus de alto risco, tanto em LSIL quanto em HSIL. Houve persistência viral em 83,3% dos casos de seguimento, sendo que em todos os casos (100%) houve a presença de HPV de alto risco oncogênico, fosse de forma isolada ou associado à infecção por vírus de baixo risco. Conclusões: As LICs estão frequentemente associadas ao HPV, sendo o grupo de alto risco mais comumente encontrado, com cargas virais altas e muito altas, demonstrando uma tendência à sua elevação de acordo com a severidade da LIC encontrada na citologia. Houve persistência viral em 83,3% dos casos com seguimento, com a presença do HPV de alto risco em todos os casos.
- ItemAcesso aberto (Open Access)Association between human Papillomavirus and colorectal adenocarcinoma and its influence on tumor staging and degree of cell differentiation(Colégio Brasileiro de Cirurgia Digestiva, 2014-09-01) Picanço-Junior, Olavo Magalhães; Oliveira, Andre Luiz Torres; Freire, Lucia Thereza Mascarenhas; Brito, Rosangela Baia; Villa, Luisa Lina; Matos, Delcio [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Ophyr Loiola Hospital; Institute at the Oswaldo Cruz German HospitalBACKGROUND:Colorectal cancer is one of the most common types of neoplasia among the worldwide adult population. Among neoplasms of the gastrointestinal tract, it is ranked second in relation to prevalence and mortality, but its etiology is only known in around 5% of the cases. It is believed that 15% of malignant diseases are related to viral oncogenesis.AIM:To correlate the presence of HPV with the staging and degree of cell differentiation among patients with colorectal adenocarcinoma.METHODS:A retrospective case-control study was conducted on 144 patients divided between a test group of 79 cases of colorectal cancer and a control group to analyze 144 patients aged 25 to 85 years (mean, 57.85 years; standard deviation, 15.27 years and median, 58 years). Eighty-six patients (59.7%) were male. For both groups, tissue samples from paraffin blocks were subjected to DNA extraction followed by the polymerase chain reaction using generic and specific primers for HPV 16 and 18. Dot blot hybridization was also performed with the aim of identifying HPV DNA.RESULTS:The groups were shown to be homogenous regarding sex, age and site of HPV findings in the samples analyzed. Out of the 41 patients with HPV, 36 (45.6%) were in the cases and five (7.7%) were in the control group (p<0.001). All the HPV cases observed comprised HPV 16, and HPV 18 was not shown in any of the cases studied. There were no significant differences in comparisons of sex, age and site regarding the presence of HPV in either of the groups. It was not observe any significant difference in relation to staging or degree of cell differentiation among the patients with colorectal cancer.CONCLUSION:Human papillomavirus type 16 is present in individuals with colorectal carcinoma. However, its presence was unrelated to staging or degree of differentiation.
- ItemSomente MetadadadosEffectiveness of the human papillomavirus (types 6, 11, 16, and 18) vaccine in the treatment of children with recurrent respiratory papillomatosis(Elsevier Ireland Ltd, 2016) Hermann, Juliana Sato [UNIFESP]; Weckx, Lily Yin [UNIFESP]; Nurmberger, Jussimara Monteiro; Santos Junior, Gildo Francisco dos; Pignatari, Antonio Carlos Campos [UNIFESP]; Pignatari, Shirley Shizue Nagata [UNIFESP]Objective: To evaluate whether the quadrivalent human papillomavirus (HPV) (types 6, 11, 16, and 18) vaccine influences the clinical course of juvenile-onset recurrent respiratory papillomatosis (RRP) when administered to a group of patients with this condition. Methods: Uncontrolled intervention study of patients with juvenile-onset RRP examined at the Pediatric Otorhinolaryngology Clinic, Federal University of Sao Paulo, where nine patients between the ages of nine and 17 received three doses of the prophylactic quadrivalent HPV vaccine (Gardasil) and were followed for one year. Disease staging, intervals between relapses, intervals between surgeries, and the number of surgeries during the year prior to vaccination and during the first year after vaccination were compared. Results: Eight patients were infected with HPV-6 and one with HPV-11. There were no statistically significant differences in the clinical scores (p = 0.083), anatomical scores (p = 0.257), intervals between relapses (p = 0.062), intervals between surgeries (p = 0.357), or the numbers of surgeries (p = 0.180) when the years before and after vaccination were compared. All patients had relapses following vaccination. Conclusion: Patients with juvenile-onset RRP experienced a similar clinical course in the year after versus the year before vaccination with Gardasil. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
- ItemAcesso aberto (Open Access)Expressão de topoisomerase II alfa e de caspase-3 ativada em lesão intra-epitelial cervical escamosa de baixo grau(Universidade Federal de São Paulo, 2008-03-26) Coelho, Raquel Autran [UNIFESP]; Ribalta, Julisa Chamorro Lascasas [UNIFESP]; Universidade Federal de São Paulo [UNIFESP]Purpose: To evaluate the correlation between the expression of topoisomerase II alpha, active caspase-3 and infection with human papillomavirus in low-grade cervical intraepithelial lesion and in the normal cervix, and whether they might influence susceptibility to, or evolution of, cervical lesion. Patients and methods: Forty cervical biopsies patients with low-grade cervical intraepithelial lesion and thirty-two with normal cervix were stained by immunohistochemistry for topoisomerase IIá and active caspase-3 and were investigated for the presence of HPV on exfoliated cells by general primer GP5+/6+ PCR amplification of DNA. These findings were correlated with clinicopathological features of the patients including their clinical outcome after twelve months. Subjects provided written informed consent. Results: Low-grade CIN patients as a group had a significantly higher expression of topoisomerase II alpha compared to controls, without correlation to disease outcome at 12 months. Caspase-3 was expressed in 42.5% of CIN patients and in 15.63% without disease, and most of women without caspase-3 receded cervical lesion. HPV DNA testing was positive in 65% of the patients with cervical lesion, and in 59.4% of the control group and was not associated to the expression of topoisomerase IIá or active caspase-3. In the presence of a positive HPV DNA testing, women with cervical lesion had a significantly higher expression of topoisomerase II alpha compared to controls. Conclusion: Topoisomerase II alpha and active caspase-3 might be useful diagnostic and prognostic markers in low-grade cervical lesions, delaying a better follow-up.
- ItemAcesso aberto (Open Access)Influência do uso de anticoncepcionais hormonais orais sobre o número de células de Langerhans em mulheres com captura híbrida negativa para papilomavírus humano(Federação Brasileira das Sociedades de Ginecologia e Obstetrícia, 2005-12-01) Uchimura, Nelson Shozo [UNIFESP]; Ribalta, Julisa Chamorro Lascasas [UNIFESP]; Focchi, José [UNIFESP]; Baracat, Edmund Chada [UNIFESP]; Uchimura, Taqueco Teruya; Universidade Estadual de Maringá Divisão de Ginecologia e Obstetrícia do Hospital Universitário de Maringá Departamento de Medicina; Universidade Federal de São Paulo (UNIFESP); Universidade Estadual de MaringáPURPOSE: to study the influence of the use of oral contraceptives (OC) on the number of Langerhans' cells in women without cervical infection by human papillomavirus (HPV). METHODS: thirty women who presented abnormal cervical cytology and colposcopy-guided biopsy with samples of uterine cervix negative for HPV were selected. The absence of HPV DNA was confirmed by hybrid capture. Langerhans' cells were identified by immunohistochemistry using anti-S100 antigens. The cells visualized in light microscopy were counted using the Cytoviewer software. The nonparametric Wilcoxon rank sum test was employed for statistical analysis. RESULTS: the average number of Langerhans' cells in OC users was 320.7/mm² and in non-users 190.7/mm², this difference being statistically nonsignificant. In the intermediary layer of the cervical epithelium a tendency towards the increase of these cells was observed, with the averages 192.1/mm² for OC users and 93.4/mm² for non-users (p=0.05). CONCLUSIONS: the present study reports a tendency towards the increase in the number of the Langerhans' cells among OC users. This result suggests the OC may induce alterations in the number of Langerhans' cells, but considering the limited number of cases, more studies should be developed for a definitive conclusion.
- ItemAcesso aberto (Open Access)Prevalence of sexually transmitted diseases in female athletes in São Paulo, Brazil(Instituto Israelita de Ensino e Pesquisa Albert Einstein, 2014-03-01) Araujo, Maíta Poli de; Kleine, Henrique Truffa; Parmigiano, Tathiana Rebizzi; Gomes, Natalia Tavares; Caparroz, Graziela Pascom; Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]; Girão, Manoel João Batista Castello [UNIFESP]; Sartori, Marair Gracio Ferreira [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objective: To determine the prevalence of sexually transmitted diseases in female athletes. Method: An observational, cross-sectional study was conducted including 50 female athletes with mean age of 20±3 years. Colposcopy, pap smear, and polymerase chain reaction for Chlamydia trachomatis, human papillomavirus and Neisseria gonorrhoeae were performed. Blood samples were collected to test for the human immunodeficiency virus, syphilis, hepatitis B and C. The athletes presenting clinical diseases or conditions identifiable by laboratory tests were treated and followed up in the unit. Result: Forty-six percent of the participants were unaware of sexually transmitted diseases. The prevalence of sexually transmitted diseases among athletes was 48% (24 cases). Human papillomavirus was the most frequent agent (44%). Considering the human papillomavirus genotypes, subtype 16 was the most prevalent (53%), followed by 11-6 (22%) and 18 (13%). Two athletes tested positive for C. trachomatis. There were no cases diagnosed of infection by N. gonorrhoeae, syphilis, hepatitis B, hepatitis C and human immunodeficiency virus. However, only 26 athletes had been vaccinated for hepatitis B. Conclusion: The prevalence of sexually transmitted diseases in female athletes was high. Primary prevention measures (hepatitis B and human papillomavirus vaccination) and secondary (serology, pap smears) must be offered to this specific group of women. The matter should be further approached in sports.