Navegando por Palavras-chave "Papilomavirus"

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    Análise comparativa do tratamento do condiloma acuminado anal e perianal utilizando plasma de argônio e eletrofulguração em doentes HIV positivo e negativo
    (Universidade Federal de São Paulo (UNIFESP), 2017-07-31) Braga, Eduardo Angelo [UNIFESP]; Lopes Filho, Gaspar de Jesus [UNIFESP]; Saad, Sarhan Sydney [UNIFESP]; http://lattes.cnpq.br/8646840760424911; http://lattes.cnpq.br/3518607824692081; http://lattes.cnpq.br/2072176749270040; Universidade Federal de São Paulo (UNIFESP)
    Introduction: Human papillomavirus (HPV) infection is the sexually transmitted disease most frequently diagnosed in Brazil, in both men and women and its incidence has been increasing over recent years. It may occur in association with the human immunodeficiency virus (HIV) in up to 62% of the cases1,2. Despite various forms of treatment for Condylomata, relapses are frequent and both the immediate and the long-term results are unsatisfactory. Use of argon plasma is an attractive option because its results are effective. However, further studies comparing this with the standard treatment are still needed. Purpose: To compare the efficiency and complications of treatments for anal and perianal condyloma using argon plasma and electrofulguration and the recurrence rates of these treatments. Methods: From January 2013 to April 2014, in 37 cases of perianal and anal condyloma, the patients’ anal region was divided into two semicircles. Treatment with argon plasma coagulation or electrofulguration was then randomly assigned (one method for each semicircle). The therapeutic sessions were repeated until clinical signs of HPV infection had been eliminated. Tissue samples were taken for cytological, histological and PCR evaluations. Results: The HPV genotype, presence of more than one genotype per patient, oncological potential per genotype and cytological and histological results were analyzed. There was no significant difference in effectiveness between the argon and fulguration methods, based on recurrence and disease-free interval (p > 0.05). In relation to argon application, the variables studied were not associated with recurrence of condyloma (p > 0.05). However, the areas treated with electrofulguration in HIV-positive patients presented more relapses, with a significant difference (p = 0.01). Conclusion: Use of argon plasma was less influenced by presence of HIV than was use of electrofulguration. With regard to efficacy, the methods were statistically equivalent, however, the areas treated with electrofulguration in HIV-positive patients presented worse results with more relapses.
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    Delineamento de um banco de primers para genotipagem e avaliação do estado físico do vírus do papiloma humano(HPV)em lesões escamosas intraepiteliais cervicas
    (Universidade Federal de São Paulo (UNIFESP), 2009-03-25) Pessoa, Felipe Barbosa [UNIFESP]; Lindsey, Charles Julian [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    The biological diversity of human papilloma virus (HPV) reflected by the diverse genetic structure of uncountable Papovaviridae viral types and a complex biological cycle, with loss of genomic encoding regions when integrating to host cell genome, poses serious challanges to identification of viral types as to the study of gene structure and pathogenic potential relationships. Hence, we chose to produce a primer bank consisting of the genomic regions pertaining to not only the better studied viral types as HPV 16 and 18 but to as many possible others, including leser studued types as HPV 33, 35 or 52. The latter retain significant biological and clinical relevance to HPV 16. Yet, to the present diagnostic methods are a limiting factor of HPV research. Presently the most promisimng options are the genotyping kits that rely on multiplex amplification reactions with generic primers and subsequent fragment hibridizing with sequence specific DNA probes. However genotyping, as is nowerdays, may be insuficient for epidemiological, prophilatic or even medical reaearch purposes. However, the most signnificant limitation of present day genotyping is that HPV genome gene prospecting is limited to a reduced portion of the known viral types, ignoring all other HPV types irrespective of their oncogenic potential. Further more, identification of gene expression for gene structure and pathogenicity studues is not an option with the latter approach. Hence, the present proposal for HPV prevalence and host genome studies hinges on 2 approaches. One aims at producing a primer bank for identifying individual HPV genomes, gene and gene product expression for the widest range of virus types possible, while the other aims at the conception of generic primers sets that in combination with the selective primers may yield an effective system for HPV prevalence in given populations as well as the possibility of fathoming oncogenic activity in infected patients. The development of diagnostic kits with expanded sensitivity matching the diversity of the Papovaviridae family members associated to feminine genital tract oncology would be welcomed by the clinical and scientific establishment.