Navegando por Palavras-chave "SHR Rat"

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    Investigação da herança intergeracional - via espermatozoides - de alterações na expressão de miRNAs relacionados a transtornos psiquiátricos
    (Universidade Federal de São Paulo (UNIFESP), 2020-09-30) Campagnoli, Gabriella Mesas [UNIFESP]; Teixeira, Taiza Stumpp [UNIFESP]; Universidade Federal de São Paulo
    Introduction: Epigenetics is identified as an important component of the inheritance of several conditions, among which “missing heritability”, such as schizophrenia, since the genetic aspects of this disorder do not explain its heritability. Besides, the fact that the environment is crucial for the manifestation and development of schizophrenia brings to light the importance of the involvement of epigenetic mechanisms in this disorder. As schizophrenia is considered a developmental disorder, or the use of experimental models that allow investigating the development of this disorder from the early life is essential,especially with regard to respect for its molecular aspects. MiRNAs play an important role in neurogenesis, involved in behavioral changes and psychiatric disorders. Thus, an epigenetic analysis of brain regions and sperm can contribute to the understanding of epigenetic aspects involved in schizophrenia and specific about their epigenetic inheritance. Aims: To investigate the profile of miRNAs related to schizophrenia in sperm, prefrontal cortex and hippocampus of SHR rats. Methods: Sperm miRNAs, prefrontal cortex and hippocampus were extracted, quantified and reproduced in the construction of libraries for further sequencing. The captured data were used for bioinformatics and statistical analyzes, as well as an interactive analysis was carried out between differently expressed miRNAs and target genes through the Metascape and Cytoscape platforms. Results: We observed differential expression of miRNAs in the three types of tissue/cell. A bioinformatics analysis has shown that the vast majority of differently expressed miRNA target genes, both for sperm and the prefrontal cortex and hippocampus, are using the nervous system and its important pathways to relate to behavior, neurodevelopment and synaptic processes. Among the genes included, the miRNAs differently expressed, 12 coincide with the genes expressly expressed in patients with schizophrenia, or that reinforce SHR as a good model for the study of these disorders. Conclusion: the male rats of the SHR lineage show alterations in the expression of miRNAs related to neurological involvement and schizophrenia in sperm, prefrontal cortex and hippocampus, as the SHR model is useful for epigenetic studies and other molecular aspects related to schizophrenia. Finally, this study suggests that the SHR model may be useful for future studies on the epigenetic inheritance of miRNAs.