Navegando por Palavras-chave "TNF-alfa"

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    Fibroblastos periodontais humanos em meio de cultura suplementado com IL-1ß no modelo de força compressiva estática continua
    (Universidade Federal de São Paulo (UNIFESP), 2016-06-30) Miguel, Delaini Pires Roman [UNIFESP]; Ferreira, Lydia Masako [UNIFESP]; http://lattes.cnpq.br/1619822351741819; http://lattes.cnpq.br/7023262511430596; Universidade Federal de São Paulo (UNIFESP)
    Introduction: The mechanotransduction may be a cellular differentiation mechanism for fibroblasts that are derived from the periodontal ligament as well as local inflammation may also play an important role in the molecular response of these cells. Objective: Evaluate the viability, apoptosis, necrosis and gene expression of proinflammatory markers in a continuous static compression model using a proinflammatory cell culture. Methods: We selected 10 patients that were submitted to third molars extractions to obtain 4 mm2 of periodontal tissue of the middle third of their roots. Fibroblasts were isolated and expanded until the 6th passage and cultured in proinflammatory culture (IL- 1beta 5 ng / mL ) and after this three groups were formed according to the applied load: Control Group (CG), no charge; Experimental Group 1 (SG1), continuous static compression of 3g/6h and Experimental Group 2 (SG2) continuous static compression 4g/6h. Results: The cell viability control group had higher results both experimental groups, and was not observed differences between experimental groups. The cell apoptosis was higher in the experimental group 2 compared to the control and experimental groups 1, with no differences between them. No differences were observed between the groups in relation to cell necrosis. In gene expression not detected in the TNF-alpha expression, but IL-6 expression, this expression equal among all groups. Conclusion: Human periodontal fibroblasts in culture medium supplemented with IL-1 beta in continuous static compressive force model showed decreased cell viability, increased apoptosis and necrosis showed no changes and gene expression of IL-6 and TNF-alpha.
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    O papel da deficiência colinérgica na mucosa bucal de camundongos expostos à fumaça do cigarro
    (Universidade Federal de São Paulo (UNIFESP), 2020-10-06) Sousa, Thamires Cristina Furlanetti de [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Moura, Carolina Foot Gomes de [UNIFESP]; http://lattes.cnpq.br/7337258146927440; http://lattes.cnpq.br/9969803499258672; http://lattes.cnpq.br/6055311539909897; Universidade Federal de São Paulo (UNIFESP)
    O tabagismo constitui um grande problema de Saúde Pública por participar da patogênese de diversas doenças crônico-degenerativas, incluindo o câncer. A acetilcolina é um neurotransmissor capaz de inibir a resposta inflamatória por meio da via anti-inflamatória colinérgica, cuja deficiência na vesícula transportadora de acetilcolina (VAChT) resulta em um estado pró-inflamatório sistêmico. Neste estudo, foi avaliada a expressão de marcadores inflamatórios e de proliferação celular na mucosa bucal de camundongos com deficiência de expressão do VAChT e expostos à fumaça do cigarro. Para tanto, 8 camundongos machos selvagens/wild type (WT) e 8 camundongos machos deficientes (knockdown, KD) para VAChT foram distribuídos em 4 grupos experimentais (n=4): Grupo WT - Camundongos selvagens / wild-type (controle) não expostos à fumaça de cigarro; Grupo KD - Camundongos com deficiência de expressão do VAChT não expostos à fumaça de cigarro; Grupo WT+Fumo - Camundongos selvagens / wild-type expostos à fumaça de cigarro por 12 semanas e Grupo KD+Fumo - camundongos com deficiência de expressão de VAChT expostos à fumaça de cigarro por 12 semanas. Os resultados da análise do marcador inflamatório TNF-alfa apontaram diferença estatística em todos os grupos experimentais quando comparados ao grupo controle (WT). Na análise imunoistoquímica para o marcador ki-67 foi observado que os animais do grupo controle e expostos ao fumo (grupo WT + FUMO) apresentaram um aumento na proliferação celular quando comparados aos animais dos grupos WT e KD. A análise histopatológica da língua dos animais mostrou que o grupo KD+ FUMO apresentou redução no número de focos de displasia epitelial comparado aos animais dos grupos KD e WT + FUMO. Em suma, tais resultados sugerem que a exposição à fumaça do cigarro é capaz de induzir aumento da atividade inflamatória e proliferação celular, sendo dependente da presença da acetilcolina.
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    Uso de corticóide como inibidor da resposta inflamatória sistêmica induzida pela circulação extracorpórea
    (Sociedade Brasileira de Cirurgia Cardiovascular, 1999-07-01) Brasil, Luiz Antonio; Gomes, Walter José [UNIFESP]; Salomão, Reinaldo [UNIFESP]; Fonseca, José Honório de Almeida Palma da [UNIFESP]; Branco, João Nelson Rodrigues [UNIFESP]; Buffolo, Enio [UNIFESP]; Universidade Federal de Goiás; Universidade Federal de São Paulo (UNIFESP)
    Cardiopulmonary bypass (CPB) induces the development of a systemic inflammatory response syndrome, with the release of cytokines that are responsible for many clinical manifestations. PURPOSE: The purpose of the study was to observe the release of the cytokines - tumor necrosis factor alpha (TNFa) and Interleukine-6 (IL-6), and to verify the clinical alterations produced in patients undergoing myocardial revascularization with CPB, with or without corticoids. MATERIAL AND METHODS: Thirty patients were studied - 15 used corticoid (methylprednisolone, 30 mg/kg -Group I) and 15 did not (Group II). Serial blood samples were collected and the TNFa and IL-6 release were analyzed, as well as the leukocyte count, erythrocyte sedimentation rate and glycemia. The blood pressure, cardiac rate, temperature, postoperative bleeding, orotracheal tubing time and inotropic drug requirement were also compared. Statistical significance was assumed when p £ 0.05. RESULTS: In Group I TNFa was not detected and IL-6 was detected in 13 patients, with levels ranging from 8.6 to 101.8 pg/ml. In Group II TNFa was detected in 13 patients, with levels between 5.4 and 231.0 pg/ml. The IL-6 in this group was detected in 15 patients, with higher levels than those in Group I, varying between 5.5 and 2569.0 pg/ml. The Group I patients had higher medium blood pressure (7.9 ± 0.5 vs 7.3 ± 0.4 mmHg) and lower inotropic drug requirement (5 vs 11). They evolved with less tachycardia (105.6 ± 5.9 vs 109.3 ± 7.2 bpm), lower temperature (36.5 ± 0.2 vs 37.3 ± 0.2°C), lower postoperative bleeding, (576.6 ± 119.5 vs 810.0 ± 176.2 ml), shorter orotracheal tubing time (11.0 ± 2.0 vs 14.6 ± 2.9 hs) and lower leukocytosis. The glycemia level was just significant (Group I > Grupo II) in the immediate postoperative and in the first postoperative samples. The erythrocyte sedimentation rate did not present significant statistical difference between the two groups. CONCLUSION: The methylprednisolone significantly inhibited the release of inflammatory cytokines mainly the TNFa. The systemic adverse effects caused by the inflammatory response after CPB were minimized by corticoid use.