Navegando por Palavras-chave "cryptococcus neoformans"

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    Avaliação dos gens atg8,ams1 e hrr25 do patógeno cryptococcus neoformans: impactos sobre os fatores de virulência e autofagia
    (Universidade Federal de São Paulo (UNIFESP), 2016-08-31) Lima, Ricardo Ferreira [UNIFESP]; Pascon, Renata Castiglioni Pascon [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Invasive fungal infections (IFES) increase exponentially in recent years. The IFIs are difficult to treat due to limited options of antifungals, especially cryptococcosis, which presents itself as an infection that causes high mortality in immunocompromised individuals affected by AIDS, transplant or undergoing chemotherapy. The etiological agent of this disease; Cryptococcus neoformans is an opportunistic yeast that besides the medical importance, it is also an excellent biological study model. This micro-organism is easy to grow and genetically manipulate that enable the analysis of the molecular attributes related to pathogenesis. C. neoformans expressed many virulence factors during the infection process, among these, the thermotolerance that enables it to develop at 37°C. In this yeast it was isolated and characterized the mutant ape4 unable to grow at 37°C. The APE4 protein is involved in Cytoplasm to Vacuole Targeting (Cvt) process and autophagy, in Saccharomyces cerevisiae. In S. cerevisiae there are seven proteins involved in the Cvt process (HRR25, ATG8, ATG11, ATG19, APE1, APE4 e AMS1). Using these S. cerevisiae sequences to browse the C. neoformans database at the Broad Institute, we found only 4 sequences (ATG8, AMS1 e HRR25) that are homolog to S. cereivisiae. Therefore, the objective of this study is to explore the biological function of the 3 proteins in C. neoformans relating them to the capacity of this yeast grow at physiological temperature of mammals and autophagy. Moreover, the study of HRR25 coding gene will be further explored using the Degron technique, which is currently being established in C. neoformans.