Navegando por Palavras-chave "progesterone"
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- ItemSomente MetadadadosThe anxiolytic effect of pregnancy in rats is reversed by finasteride(Elsevier B.V., 2006-11-01) Faturi, Claudia de Brito; Teixeira-Silva, Flavia; Leite, Jose Roberto; Universidade Federal de Sergipe (UFS); Universidade Federal de São Paulo (UNIFESP)Several studies have shown the influence of the oestrous cycle on anxiety levels and the important role of progesterone in this effect. the metabolism of this steroid hormone yields neuroactive steroids among them allopregnatiolone (alloP) and allotetrahydrodeoxycorticosterone (alloTHDOC), which bind to GABA(A) receptors and have anxiolytic effects. Considering that during pregnancy there is an increase in levels of both progesterone and its metabolites, the main objectives of this work were: (1) to assess changes in anxiety levels during pregnancy and (2) to verify the role of alloP and alloTHDOC in this process using finasteride, an inhibitor of 5 alpha-reductase, the enzyme responsible for their synthesis.The results showed a significant reduction in anxiety levels on the 19th day of pregnancy, which was reversed by finasteride, suggesting a role for alloP and alloTHDOC in the anxiolytic process. (c) 2006 Elsevier Inc. All rights reserved.
- ItemSomente MetadadadosConcentration of sulfated glycosaminoglycans in the mammary tissue of female rats with the aging and about hormonal influence(Taylor & Francis Ltd, 2018) Torres, Sueli M. P. S. [UNIFESP]; Nader, Helena B. [UNIFESP]; Simoes, Ricardo S. [UNIFESP]; Baracat, Edmund C. [UNIFESP]; Simoes, Manuel de J. [UNIFESP]; Fuchs, Luiz F. P. [UNIFESP]; Soares-, Jose Maria, Jr. [UNIFESP]; Gomes, Regina Celia T. [UNIFESP]It was to evaluate the concentration of sulfate glycosaminoglycans (GAG) in mammary tissue of the young and adult female rats and ovariectomized females rats after hormonal stimulation. For this purpose, 60 female rats were divided into six groups with 10 animals/each: nonovariectomized groups: G1 (5 months), and G2 (15 months) and ovariectomized groups: OG (vehicle)
- ItemSomente MetadadadosEffects of progesterone on sleep: A possible pharmacological treatment for sleep-breathing disorders?(Bentham Science Publ Ltd, 2006-01-01) Andersen, Monica Levy [UNIFESP]; Bittencourt, Lia Rita Azeredo [UNIFESP]; Antunes, I. B.; Tufik, Sergio [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Progesterone is present in a wide spectrum of biological activity within a variety of tissues. This hormone is also known to affect reproduction, sleep quality, respiration, mood, appetite, learning, memory and sexual activity. Progesterone exerts a sleep induction or hypnotic effect and is a potent respiratory stimulant that has been associated to a decrease in the number of central and obstructive sleep apnea episodes in men. the literature also contains a substantial amount of data on the effect of apnea in women with obesity-hypoventilation during menopause. This review attempts to outline the specific role of progesterone in normal sleep and breathing as well as its possible therapeutic effects in the treatment of sleep-disordered breathing.
- ItemSomente MetadadadosHormonal profile, the PROGINS polymorphism, and erectile dysfunction complaints: data from a population-based survey(Elsevier B.V., 2011-02-01) Andersen, Monica L. [UNIFESP]; Alvarenga, Tathiana A. [UNIFESP]; Mazzotti, Diego R. [UNIFESP]; Guindalini, Camila [UNIFESP]; Pellegrino, Renata [UNIFESP]; Barrueco, Karina F. [UNIFESP]; Santos-Silva, Rogerio [UNIFESP]; Bittencourt, Lia R. [UNIFESP]; Tufik, Sergio [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objective: To evaluate a potential association among the hormonal profile, PROGINS polymorphism, and erectile dysfunction (ED) complaints in a large population-based sample in São Paulo, Brazil.Design: Population-based questionnaire study.Setting: Interviews, sleep recording, and blood sample were conducted in a sleep institute.Patient(s): the total study participants included 467 men.Intervention(s): General information was obtained through interviews, and a blood sample was collected for hormone levels, DNA extraction, and PROGINS genotyping.Main Outcome Measure(s): the effect of progesterone and the PROGINS polymorphism on the risk of developing ED were measured by questionnaire and blood sample.Result(s): Progesterone, prolactin, testosterone, and estradiol levels did not differ between the genotype groups (T1/T1 and T1/T2+T2/T2). No significant genotypic or allelic differences were found between individuals with ED complaints and controls. Multivariate logistic regression analyses including age, body mass index, hypertension, diabetes, apnea-hypopnea index, and genetic ancestry estimation, as well as the PROGINS polymorphism, confirmed the lack of association between the T2 allele carriers and the risk of ED(odds ratio = 0.80; 95% confidence interval = 0.40-1.62).Conclusion(s): This is the first study to demonstrate the genotypic and allelic frequencies of the PROGINS polymorphism in a large population-based sample of men. the results do not support a direct role for the PROGINS polymorphism in the risk of developing ED; however, further examination of other variants within PR gene will be necessary to completely rule out an effect. (Fertil Steril (R) 2011;95:621-4. (C) 2011 by American Society for Reproductive Medicine.)
- ItemSomente MetadadadosImpairment of male reproductive function after sleep deprivation(Elsevier B.V., 2015-05-01) Alvarenga, Tathiana A. [UNIFESP]; Hirotsu, Camila [UNIFESP]; Mazaro-Costa, Renata; Tufik, Sergio [UNIFESP]; Andersen, Monica L. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade Federal de Goiás (UFG)Objective: To evaluate the influence of sleep loss on sexual behavior, hormone levels, sperm parameters, and testis-specific gene expression in male rats.Design: Experimental research.Setting: Animal laboratory.Animal(s): Male adult Wistar-Hannover rats.Intervention(s): Sexually experienced rats were subjected to paradoxic sleep deprivation (PSD) for 96 hours or sleep restriction (SR) for 21 days or kept in their home cage as control (CTRL).Main Outcome Measure(s): Sexual behavior, hormone levels, sperm parameters and expression of stress and nitric oxide-related genes were evaluated.Result(s): PSD significantly decreased sexual behavior compared with the CTRL group, whereas SR had no effect. the PSD group had significantly lower testosterone levels than the CTRL group. Both PSD and SR groups had lower sperm viabilities than the CTRL group. the decrease in the number of live sperm compared with the CTRL group was larger in the PSD group than in the SR group. Regarding testicular gene expression, both PSD and SR led to an increase of iNOS and hydroxysteroid 11 beta-dehydrogenase 1 expressions compared with the CTRL group. These changes were more pronounced in the PSD group. A significant increase in endothelial nitric oxide synthase expression was observed in the PSD groups compared with the CTRL group. No changes were observed in dimethylarginine dimethylaminohydrolase 1 and casein kinase 2 beta-polypeptide expressions.Conclusion(s): Sleep loss can promote marked changes in the male reproductive system of rats, particularly affecting spermatic function in part by interfering in the testicular nitric oxide pathway. (C) 2015 by American Society for Reproductive Medicine.
- ItemSomente MetadadadosOral stomatitis induced by endogenous progesterone: Case report(Informa Healthcare, 2009-01-01) Minicucci, Eliana M.; Carrenho, Aline B.; Weber, Silke A. T.; Bombini, Fernanda M.; Ribeiro, Renata A. M. A.; Marques, Mariangela E. A.; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Oral stomatitis induced by endogenous progesterone is a rare clinical condition which may be associated with cutaneous involvement. That is probably due to the peak of progesterone production during the luteal phase of the menstrual cycle. in the present case report, a 21-year-old patient displayed recurrent ulcerative lesions located on the buccal mucosa or the upper lip, on a monthly basis since the age of 15. Such lesions would always manifest themselves on the second day until the end of the menstrual cycle.
- ItemSomente MetadadadosOvarian-Steroid Modulation of Locus Coeruleus Activity in Female Rats: Involvement in Luteinising Hormone Regulation(Wiley-Blackwell, 2009-07-01) Szawka, R. E.; Rodovalho, G. V.; Monteiro, P. M.; Carrer, H. F.; Anselmo-Franci, J. A. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP); Consejo Nacl Invest Cient & TecnThe noradrenergic nucleus locus coeruleus (LC) has been reported to regulate luteinising hormone (LH) secretion in female rats. Both oestrogen and progestin receptors have been demonstrated in LC neurones, suggesting that these cells are possibly responsive to variations in circulating levels of ovarian steroids. We therefore evaluated changes in the activity of LC neurones during the oestrous cycle and after ovarian-steroid treatment in ovariectomised (OVX) rats, as determined by immunoreactivity to Fos-related antigens (FRA), which comprises all of the known members of the Fos family. Effects of ovarian steroids on the firing rate of LC neurones were also determined in a slice preparation. the number of FRA/tyrosine hydroxylase (TH)-immunoreactive (ir) neurones in the LC increased from 14.00-16.00 h on pro-oestrus, coinciding with the onset of the LH surge and rise in plasma progesterone. FRA immunoreactivity was unaltered during dioestrus. Oestradiol-treated OVX rats (OVX+E) displayed marked reduction in FRA/TH-ir neurones in LC compared to oil-treated OVX rats. Accordingly, oestradiol superfusion significantly reduced the spontaneous firing rate of LC neurones in slices from OVX rats. Compared to OVX+E, oestradiol-treated rats injected with progesterone at 08.00 h (OVX+EP) exhibited higher number of FRA/TH-ir neurones in the LC at 10.00 h and 16.00 h, and great amplification of the LH surge. Bath application of progesterone significantly increased the spontaneous firing rate of OVX+E LC neurones. Our data suggest that ovarian steroids may physiologically modulate the activity of LC neurones in females, with possible implications for LH secretion. Moreover, oestradiol and progesterone appear to exert opposite and complementary effects (i.e. whereas oestradiol inhibits, progesterone, after oestradiol priming, stimulates LC activity).
- ItemSomente MetadadadosProgesterone induces apoptosis of insulin-secreting cells: insights into the molecular mechanism(Bioscientifica Ltd, 2014-05-01) Nunes, V. A.; Portioli-Sanches, E. P.; Rosim, M. P.; Araujo, M. S. [UNIFESP]; Praxedes-Garcia, P. [UNIFESP]; Valle, M. M. R.; Roma, L. P.; Hahn, C.; Gurgul-Convey, E.; Lenzen, S.; Azevedo-Martins, A. K.; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP); Hannover Med SchProgesterone has been associated with the development of gestational diabetes (GD) due to the enhancement of insulin resistance. As b-cell apoptosis participates in type 1 and type 2 diabetes pathophysiology, we proposed the hypothesis that progesterone might contribute to the development of GD through a mechanism that also involves b-cell death. To address this question, RINm5F insulin-producing cells were incubated with progesterone (25-100 mM), in the presence or absence of a-tocopherol (40 mM). After 24 or 48 h, membrane integrity andDNA fragmentation were analyzed by flow cytometry. Caspase activity was used to identify the mode of cell death. the involvement of endoplasmic reticulum stress in the action of progesterone was investigated by western blotting. Oxidative stress was measured by 2', 7'-dichlorofluorescein diacetate (DCFDA) oxidation. Isolated rat islets were used in similar experiments in order to confirm the effect of progesterone in primary b-cells. Incubation of RINm5F cells with progesterone increased the number of cells with loss of membrane integrity and DNA fragmentation. Progesterone induced generation of reactive species. Pre-incubation with a-tocopherol attenuated progesterone-induced apoptosis. Western blot analyses revealed increased expression of CREB2 and CHOP in progesteronetreated cells. Progesterone caused apoptotic death of rat islet cells and enhanced generation of reactive species. Our results show that progesterone can be toxic to pancreatic b-cells through an oxidative-stress-dependent mechanism that induces apoptosis. This effect may contribute to the development of GD during pregnancy, particularly under conditions that require administration of pharmacological doses of this hormone.
- ItemSomente MetadadadosTransvaginal ultrasonography and the progestogen challenge test in postmenopausal endometrial evaluation(Elsevier B.V., 1997-09-01) Bortoletto, CCR; Baracat, E. C.; Goncalves, Wagner Jose [UNIFESP]; Lima, G. R.; Stavale, J. N.; Universidade Federal de São Paulo (UNIFESP)Objective: To analyze the morphologic and ultrasonographic aspects of the endometrium of postmenopausal women according to the progestogen challenge test. Methods: the study was conducted on 150 postmenopausal women. Each patient was submitted to transvaginal ultrasonography for measurement of endometrial echo thickness and to endometrial biopsies, followed by the progestogen challenge test. Results: Women with a negative test presented atrophic endometrium in 94% of cases. the other 6% have shown active endometrium, but none had hyperplasia. However, 56% of the patients with a positive test had atrophic endometrium. There was a correlation between endometrial thickness less than 5 mm and endometrial atrophy in patients with either positive or negative tests. Conclusion: Because the progestogen challenge test is cheap and easy to deal with, it can be done as a primary screening method in asymptomatic postmenopausal women. If the test is positive, ultrasonography is required in order to determine who needs a more accurate examination of the endometrium. If the test is negative, ultrasonography is not required due to the great number of women who have atrophic endometrium. (C) 1997 International Federation of Gynecology and Obstetrics.