Avaliação da transmissão zoonótica do SARS-CoV-2 entre tutores infectados e seus animais de estimação: gatos e cães
Data
2023-12-10
Autores
Silva, Thiago Abranches da 
Orientadores
Cervantes, Onivaldo
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
INTRODUÇÃO: A COVID-19 (do inglês coronavírus disease 2019), causada pelo agente etiológico denominado SARS-CoV-2 (Coronavírus da Síndrome Respiratória Aguda Grave 2), surgiu em dezembro de 2019. Neste período, pacientes que tiveram quadro clínico grave apresentaram SRAG (Síndrome Respiratória Aguda Grave) e muitos deles foram à óbito. Concomitante a este período, espécies de animais domésticos chamaram atenção quando se isolou SARS-CoV-2 em gatos, pangolins e cães, em testes experimentais, e em outros casos esporádicos. Dessa forma, surgiram novos trabalhos mostrando que animais que estavam em convívio com pessoas infectadas também poderiam se infectar, porém a infecção animal/homem não foi significativamente comprovada. OBJETIVO: Avaliar a possível transmissão zoonótica do SARS-CoV-2 entre participantes/tutores infectados e seus animais de estimação como gatos e cães. MÉTODO: Foi realizado um recrutamento de pacientes que possuíam RT-PCR positivos para SARS-CoV-2, que tivessem animais de estimação, que responderam um questionário para entender a relação entre tutor/animal com agendamento de uma visita na residência para coleta de material e realização dos testes rápidos para detecção de antígeno. Foram selecionados 192 animais domésticos, sendo gatos e cães que mantiveram permanente contato com seu tutor positivos sintomáticos. Em seguida, os animais foram submetidos aos testes de antígenos RT-PCR. RESULTADOS: A análise comparativa dos parâmetros de testes rápidos aplicados para o diagnóstico de anticorpos SARS CoV-2 resultou em uma taxa de especificidade de 100% dos testes negativos para todos os testes rápidos antígenos RT-PCR aplicados em gatos e cães. CONCLUSÃO: Concluímos que apesar dos cães e gatos serem suscetíveis ao vírus SARS-CoV-2, e ao evento de transmissão zoonótica reversa, sobretudo, os gatos, não há evidências significativas de circulação do SARS-CoV-2 nas espécies. Não houve animais que apresentaram teste positivo para SARS-CoV-2 nesse método de coleta e exame.
INTRODUCTION: COVID-19 (coronavirus disease 2019), caused by the etiological agent called SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), emerged in December 2019. During this period, patients who had a severe clinical condition presented SARS (Severe Acute Respiratory Syndrome) and many of them died. Concomitant to this period, species of domestic animals drew attention when SARS-CoV-2 was isolated in cats, pangolins and dogs, in experimental tests, and in other sporadic cases. Thus, new studies have emerged showing that animals that were in contact with infected people could also become infected, but animal/human infection has not been significantly proven. OBJECTIVE: To evaluate the possible zoonotic transmission of SARS-CoV-2 between infected participants/guardians and their pets such as cats and dogs. METHOD: Patients who were RT-PCR positive for SARS-CoV-2, who had pets, who answered a questionnaire to understand the relationship between guardian/animal and scheduled a visit to the home to collect material were recruited and carrying out rapid tests for antigen detection. 192 domestic animals were selected, including cats and dogs that maintained permanent contact with their symptomatic positive owner. Then, the animals were subjected to RT-PCR antigen tests. RESULTS: The comparative analysis of the parameters of rapid tests applied for the diagnosis of SARS-CoV-2 antibodies resulted in a specificity rate of 100% of negative tests for all rapid RT-PCR antigen tests applied to cats and dogs. CONCLUSION: We conclude that although dogs and cats are susceptible to the SARS-CoV-2 virus, and to the reverse zoonotic transmission event, especially cats, there is no significant evidence of SARS-CoV-2 circulation in the species. There were no animals that tested positive for SARS-CoV-2 using this collection and examination method.
INTRODUCTION: COVID-19 (coronavirus disease 2019), caused by the etiological agent called SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), emerged in December 2019. During this period, patients who had a severe clinical condition presented SARS (Severe Acute Respiratory Syndrome) and many of them died. Concomitant to this period, species of domestic animals drew attention when SARS-CoV-2 was isolated in cats, pangolins and dogs, in experimental tests, and in other sporadic cases. Thus, new studies have emerged showing that animals that were in contact with infected people could also become infected, but animal/human infection has not been significantly proven. OBJECTIVE: To evaluate the possible zoonotic transmission of SARS-CoV-2 between infected participants/guardians and their pets such as cats and dogs. METHOD: Patients who were RT-PCR positive for SARS-CoV-2, who had pets, who answered a questionnaire to understand the relationship between guardian/animal and scheduled a visit to the home to collect material were recruited and carrying out rapid tests for antigen detection. 192 domestic animals were selected, including cats and dogs that maintained permanent contact with their symptomatic positive owner. Then, the animals were subjected to RT-PCR antigen tests. RESULTS: The comparative analysis of the parameters of rapid tests applied for the diagnosis of SARS-CoV-2 antibodies resulted in a specificity rate of 100% of negative tests for all rapid RT-PCR antigen tests applied to cats and dogs. CONCLUSION: We conclude that although dogs and cats are susceptible to the SARS-CoV-2 virus, and to the reverse zoonotic transmission event, especially cats, there is no significant evidence of SARS-CoV-2 circulation in the species. There were no animals that tested positive for SARS-CoV-2 using this collection and examination method.