Differentiation of Klebsiella pneumoniae carbapenemase (KPC) variants by pyrosequencing

Monteiro, Jussimara [UNIFESP]; Widen, Raymond H.; Pignatari, Antonio C. C. [UNIFESP]; Kubasek, Carly; Silbert, Suzane

Differentiation of Klebsiella pneumoniae carbapenemase (KPC) variants by pyrosequencing

Data

2014-05-01

Autores

Monteiro, Jussimara

Widen, Raymond H.

Pignatari, Antonio C. C.

Kubasek, Carly

Silbert, Suzane

Tipo

Artigo

Resumo

A fast and reliable protocol using the pyrosequencing technique was developed to identify 11 different types of the KPC enzyme. A total of 65 b/alpha(Kpc) positive bacterial isolates were tested and characterized. in the end, the pyrosequencing proved to be a powerful tool for epidemiological studies of KPC producer isolates. (c) 2014 Elsevier B.V. All rights reserved.

Citação

Journal of Microbiological Methods. Amsterdam: Elsevier B.V., v. 100, p. 42-45, 2014.