Differentiation of Klebsiella pneumoniae carbapenemase (KPC) variants by pyrosequencing
| dc.contributor.author | Monteiro, Jussimara [UNIFESP] | |
| dc.contributor.author | Widen, Raymond H. | |
| dc.contributor.author | Pignatari, Antonio C. C. [UNIFESP] | |
| dc.contributor.author | Kubasek, Carly | |
| dc.contributor.author | Silbert, Suzane | |
| dc.contributor.institution | Tampa Gen Hosp | |
| dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
| dc.date.accessioned | 2016-01-24T14:37:15Z | |
| dc.date.available | 2016-01-24T14:37:15Z | |
| dc.date.issued | 2014-05-01 | |
| dc.description.abstract | A fast and reliable protocol using the pyrosequencing technique was developed to identify 11 different types of the KPC enzyme. A total of 65 b/alpha(Kpc) positive bacterial isolates were tested and characterized. in the end, the pyrosequencing proved to be a powerful tool for epidemiological studies of KPC producer isolates. (c) 2014 Elsevier B.V. All rights reserved. | en |
| dc.description.affiliation | Tampa Gen Hosp, Dept Pathol, Esoter Testing Lab, Tampa, FL 33606 USA | |
| dc.description.affiliation | Universidade Federal de São Paulo, Lab Especial Microbiol Clin, São Paulo, Brazil | |
| dc.description.affiliationUnifesp | Universidade Federal de São Paulo, Lab Especial Microbiol Clin, São Paulo, Brazil | |
| dc.description.source | Web of Science | |
| dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
| dc.description.sponsorshipID | CAPES: 1152/10-6 | |
| dc.format.extent | 42-45 | |
| dc.identifier | http://dx.doi.org/10.1016/j.mimet.2014.02.013 | |
| dc.identifier.citation | Journal of Microbiological Methods. Amsterdam: Elsevier B.V., v. 100, p. 42-45, 2014. | |
| dc.identifier.doi | 10.1016/j.mimet.2014.02.013 | |
| dc.identifier.issn | 0167-7012 | |
| dc.identifier.uri | http://repositorio.unifesp.br/handle/11600/37736 | |
| dc.identifier.wos | WOS:000335631200007 | |
| dc.language.iso | eng | |
| dc.publisher | Elsevier B.V. | |
| dc.relation.ispartof | Journal of Microbiological Methods | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights.license | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
| dc.subject | Pyrosequencing | en |
| dc.subject | Real-time PCR | en |
| dc.subject | Klebsiella pneumoniae carbapenemase | en |
| dc.subject | KPC | en |
| dc.title | Differentiation of Klebsiella pneumoniae carbapenemase (KPC) variants by pyrosequencing | en |
| dc.type | info:eu-repo/semantics/article |