Molecular typing of antimicrobial-resistant Shiga-toxin-producing Escherichia coli strains (STEC) in Brazil
Data
2011-02-01
Autores
Cergole-Novella, Maria Cecilia 
Pignatari, Antonio Carlos Campos
Castanheira, Mariana
Guth, Beatriz Ernestina Cabilio
Tipo
Artigo
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Resumo
Antimicrobial resistance patterns and molecular characteristics were determined in thirty-two Shiga-toxin-producing Escherichia coli (STEC) strains previously identified in São Paulo State associated with human infections (n = 21) and in cattle feces (n = 11). the highest resistance rates were identified for tetracycline (100%), streptomycin (78%) and trimethoprim sulfamethoxazole (56%). Eleven STEC strains showed resistance to ampicillin and carried bla(TEM) that was confirmed as bla(TEM-1) in one representative isolate. the class 1 integrase gene (intI1) was detected in seven (22%) strains, and most of them belonged to the O111:H8 serotype. the class 1 integron was located on plasmids in five of the seven STEC strains, and conjugation assays confirmed the plasmid support of those resistant determinants. STEC strains were genetically classified into the B I group, and PFGE analysis showed that most of the strains in each serogroup were grouped into the same cluster (80-97% similarity). the presence of a class 1 integron and bla(TEM-1) genes is described for the first time among STEC isolates in Brazil and clearly represents a public health concern. (C) 2010 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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Citação
Research in Microbiology. Amsterdam: Elsevier B.V., v. 162, n. 2, p. 117-123, 2011.