Molecular typing of antimicrobial-resistant Shiga-toxin-producing Escherichia coli strains (STEC) in Brazil

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dc.contributor.authorCergole-Novella, Maria Cecilia [UNIFESP]
dc.contributor.authorPignatari, Antonio Carlos Campos [UNIFESP]
dc.contributor.authorCastanheira, Mariana [UNIFESP]
dc.contributor.authorGuth, Beatriz Ernestina Cabilio [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionJMI Labs
dc.date.accessioned2016-01-24T14:06:05Z
dc.date.available2016-01-24T14:06:05Z
dc.date.issued2011-02-01
dc.description.abstractAntimicrobial resistance patterns and molecular characteristics were determined in thirty-two Shiga-toxin-producing Escherichia coli (STEC) strains previously identified in São Paulo State associated with human infections (n = 21) and in cattle feces (n = 11). the highest resistance rates were identified for tetracycline (100%), streptomycin (78%) and trimethoprim sulfamethoxazole (56%). Eleven STEC strains showed resistance to ampicillin and carried bla(TEM) that was confirmed as bla(TEM-1) in one representative isolate. the class 1 integrase gene (intI1) was detected in seven (22%) strains, and most of them belonged to the O111:H8 serotype. the class 1 integron was located on plasmids in five of the seven STEC strains, and conjugation assays confirmed the plasmid support of those resistant determinants. STEC strains were genetically classified into the B I group, and PFGE analysis showed that most of the strains in each serogroup were grouped into the same cluster (80-97% similarity). the presence of a class 1 integron and bla(TEM-1) genes is described for the first time among STEC isolates in Brazil and clearly represents a public health concern. (C) 2010 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Div Infect Dis, BR-04023062 São Paulo, Brazil
dc.description.affiliationJMI Labs, N Liberty, IA USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Infect Dis, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent117-123
dc.identifierhttp://dx.doi.org/10.1016/j.resmic.2010.09.022
dc.identifier.citationResearch in Microbiology. Amsterdam: Elsevier B.V., v. 162, n. 2, p. 117-123, 2011.
dc.identifier.doi10.1016/j.resmic.2010.09.022
dc.identifier.fileWOS000288880500003.pdf
dc.identifier.issn0923-2508
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33385
dc.identifier.wosWOS:000288880500003
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofResearch in Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectShiga-toxin-producing Escherichia colien
dc.subjectResistance genesen
dc.subjectClass 1 integronen
dc.subjectPhylogenetic backgrounden
dc.titleMolecular typing of antimicrobial-resistant Shiga-toxin-producing Escherichia coli strains (STEC) in Brazilen
dc.typeinfo:eu-repo/semantics/article
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