Efeitos do 17 beta-estradiol no útero de ratas diabéticas e ovariectomizadas
Data
2015-09-30
Autores
Fonseca, Caio Cesar Navarrete da 
Orientadores
Simoes, Manuel de Jesus
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Introdução: A terapia estrogênica exerce efeitos benéficos contra os sintomas do climatério em mulheres na menopausa e em ratas ovariectomizadas (OVX). Por outro lado, estudos indicam que esses efeitos são diminuídos em modelos animais diabéticos submetidos ao tratamento com estrógeno, e assim, sugere-se uma possível influência do diabetes sobre os efeitos protetores da terapia estrogênica. Sabe-se que o estrógeno possui efeitos uterotróficos tanto em ratas OVX quanto em mulheres submetidas à reposição hormonal, no entanto, não se sabe se esses efeitos no útero são influenciados pelo diabetes. Objetivo: Avaliar os efeitos do estrógeno no útero de ratas diabéticas e ovariectomizadas. Métodos: 50 ratas adultas, com 3 meses de idade, normais ou diabéticas (Di) induzidas por estreptozotocina serão OVX (10 delas não serão OVX, apenas terão seus ovários expostos). Após 21 dias, o ciclo estral das ratas será avaliado por colpocitologia vaginal durante 4 dias consecutivos para confirmação do quadro de anestro-permanente. Posteriormente as ratas serão divididas em 5 grupos: GI (OVX-controle); GII (OVX-tratado com 10µg/Kg/dia de estrógeno); GIII (OVX-Di); GIV (OVX-Di e tratado com 10µg/Kg/dia de estrógeno); GV (SHAM-Di). As ratas serão pesadas semanalmente. Após 60 dias consecutivos de tratamento, os animais serão eutanasiados, os cornos uterinos serão coletados, pesados, fixados em formaldeído a 10% e incluídos em parafina. Cortes com 4 µm de espessura serão corados pela hematoxilina e eosina para análise histomorfométrica ou submetidos aos métodos histoquímicos de Picrosirius Red para avaliação das fibras colágenas, ao Alcian Blue (pH 0,5 e 2,5) para evidenciação e quantificação dos glicosaminoglicanos, e das análises por tricômico de Masson evidenciando colágenos. Os dados quantitativos serão submetidos à análise estatística (p<0,05).
Introduction: Estrogen therapy has beneficial effects against climacteric symptoms in menopausal women and in ovariectomized rats (OVX). On the other hand, studies indicate that these effects are decreased in diabetic animal models subjected to treatment with estrogen, and thus suggest a possible influence of diabetes on the protective effects of estrogen therapy. It is known that estrogen has uterotrophic effects in both OVX rats as in women undergoing hormone replacement, however, is not known whether these effects on the uterus are affected by diabetes. Objective: To evaluate the effects of estrogen in the uterus of diabetic rats and ovariectomized. Methods: 50 adult female rats at 3 months of age, normal or diabetic (Di) will streptozotocin induced by OVX (10 OVX them will not only have its exposed ovaries). After 21 days, the estrous cycle of the rats will be assessed by vaginal cytology for 4 consecutive days to confirm the anestrous-standing frame. Later the rats are divided into five groups: GI (OVX-control); GII (OVX-treated with 10mg / kg / day of estrogen); GIII (OVX-Di); GIV (Di-OVX and treated with 10mg / kg / day of estrogen); GV (SHAM-Di). The rats will be weighed weekly. After 60 consecutive days of treatment, the animals will be euthanized, the uterine horns will be collected, weighed, fixed in 10% formaldehyde and embedded in paraffin. Cuts with 4 mm thick are stained with hematoxylin and eosin for histomorphometric analysis or submitted to histochemistry of Sirius Red for evaluation of collagen fibers, the Alcian Blue (pH 0.5 to 2.5) for disclosure and quantification of glycosaminoglycans, and the analysis by trichrome Masson showing collagens. Quantitative data will be analyzed statistically (p <0.05).
Introduction: Estrogen therapy has beneficial effects against climacteric symptoms in menopausal women and in ovariectomized rats (OVX). On the other hand, studies indicate that these effects are decreased in diabetic animal models subjected to treatment with estrogen, and thus suggest a possible influence of diabetes on the protective effects of estrogen therapy. It is known that estrogen has uterotrophic effects in both OVX rats as in women undergoing hormone replacement, however, is not known whether these effects on the uterus are affected by diabetes. Objective: To evaluate the effects of estrogen in the uterus of diabetic rats and ovariectomized. Methods: 50 adult female rats at 3 months of age, normal or diabetic (Di) will streptozotocin induced by OVX (10 OVX them will not only have its exposed ovaries). After 21 days, the estrous cycle of the rats will be assessed by vaginal cytology for 4 consecutive days to confirm the anestrous-standing frame. Later the rats are divided into five groups: GI (OVX-control); GII (OVX-treated with 10mg / kg / day of estrogen); GIII (OVX-Di); GIV (Di-OVX and treated with 10mg / kg / day of estrogen); GV (SHAM-Di). The rats will be weighed weekly. After 60 consecutive days of treatment, the animals will be euthanized, the uterine horns will be collected, weighed, fixed in 10% formaldehyde and embedded in paraffin. Cuts with 4 mm thick are stained with hematoxylin and eosin for histomorphometric analysis or submitted to histochemistry of Sirius Red for evaluation of collagen fibers, the Alcian Blue (pH 0.5 to 2.5) for disclosure and quantification of glycosaminoglycans, and the analysis by trichrome Masson showing collagens. Quantitative data will be analyzed statistically (p <0.05).
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Citação
FONSECA, Caio Cesar Navarrete da. Efeitos do 17 beta-estradiol no útero de ratas diabéticas e ovariectomizadas. 2015. 60 f. Dissertação (Mestrado em Biologia Estrutural e Funcional) - Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP), São Paulo, 2015.