Consumo de glúten dos genitores de crianças e adolescentes com doença celíaca: avaliação quantitativa por dois métodos
Arquivos
Data
2024-02-07
Autores
Souza, Marilia Graziela Alves de 
Orientadores
Sdepanian, Vera Lucia
Tipo
Tese de doutorado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Objetivo: Quantificar o consumo de glúten dos familiares de primeiro grau dos pacientes com doença celíaca (grupo estudo) e comparar com o consumo de glúten dos familiares de primeiro grau das crianças sem restrição alimentar (grupo controle), empregando os métodos de Overbeek e de ELISA.
Métodos: Estudo caso-controle, em ambulatório especializado de gastroenterologia pediátrica, cuja entrevista abordou a frequência do consumo de alimentos com glúten, subdivididos em grupos (pães, bolos, tortas, aveia, chocolate, massas e cervejas) pelos familiares do grupo e grupo controle. Quantificou-se o consumo de cada alimento com glúten em gramas por dia, e estes foram reproduzidos, segundo as informações dos familiares. Estimou-se a quantidade de glúten em g/dia pelo método de Overbeek multiplicando-se a quantidade de proteína pelo fator 0,8. O teste de ELISA AgraQuant® Teste de ELISA Glúten G12 - laboratório Romer Labs – resultou em gramas de glúten para cada 100g do alimento.
Resultados: Participaram 36 familiares do grupo estudo e 62 familiares do grupo controle, e analisaram-se 72 alimentos. A mediana do consumo total de glúten do grupo estudo pelo método de Overbeek e ELISA foram, respectivamente, 9,10g e 13,91g, que foram estatisticamente menores que o do grupo controle, 12,58g e 26,29g. A quantidade do consumo total de glúten pelo método de Overbeek foi estatisticamente menor do que o ELISA. Os alimentos mais consumidos pelo grupo estudo e controle, segundo o ELISA foram pães e massas.
Conclusão: O consumo diário de glúten dos familiares do grupo estudo foi menor do que dos familiares do grupo controle, embora ambos grupos consumissem pães e massas com mais frequência. A redução do consumo de glúten pelo familiar do grupo estudo não repercutiu para que as crianças fossem obedientes à dieta. O uso da farinha de trigo foi muito menor nas casas dos familiares do grupo estudo do que dos familiares do grupo controle, que na quase totalidade utilizaram este ingrediente. O método estimado de Overbeek subestimou a quantidade total de glúten nos alimentos quando comparado ao ELISA.
Objective: The aim of the study was to measure gluten consumption in first-degree relatives of patients with celiac disease (study group) and compare it with gluten intake in first-degree relatives of children without dietary restrictions (control group), using both the Overbeek and ELISA methods. Methods: A case-control study was conducted in a specialized pediatric gastroenterology outpatient clinic. First-degree relatives were interviewed to assess the frequency of consumption of gluten-containing foods grouped into specific categories, such as bread, cake, pie, oats, chocolate, pasta, and beer. Gluten intake was calculated in grams per day based on the information provided by the relatives. The amount of gluten was quantified in grams per day using the Overbeek method, with the protein content multiplied by a factor of 0.8. The AgraQuant® Gluten G12 ELISA Test from Romer Labs, known as the ELISA test, was applied to determine the gluten content in grams per 100 grams of food. Results: Thirty-six family members from the study group and 62 family members from the control group participated, and 72 foods were analyzed. The median total gluten intake for the study group was 9.10 g for the Overbeek method and 13.91 g for the ELISA, significantly lower than the median total gluten intake for the control group of 12.58 g for the Overbeek method and 26.29 g for the ELISA. Moreover, total gluten consumption measured by the Overbeek method was statistically lower than that measured by ELISA. The study and control groups primarily consumed bread and pasta, as determined by ELISA analysis. Conclusions: The daily gluten consumption of family members in the study group was lower than that of family members in the control group despite both groups consuming bread and pasta more frequently. Even though first-degree relatives in the study group reduced their gluten intake, it did not impact on the adherence of their children to the diet. Wheat flour was used significantly less in the homes of the study group compared to the control group, where almost all participants had access to this ingredient. The Overbeek method detected a lower total amount of gluten in the food compared to the ELISA method.
Objective: The aim of the study was to measure gluten consumption in first-degree relatives of patients with celiac disease (study group) and compare it with gluten intake in first-degree relatives of children without dietary restrictions (control group), using both the Overbeek and ELISA methods. Methods: A case-control study was conducted in a specialized pediatric gastroenterology outpatient clinic. First-degree relatives were interviewed to assess the frequency of consumption of gluten-containing foods grouped into specific categories, such as bread, cake, pie, oats, chocolate, pasta, and beer. Gluten intake was calculated in grams per day based on the information provided by the relatives. The amount of gluten was quantified in grams per day using the Overbeek method, with the protein content multiplied by a factor of 0.8. The AgraQuant® Gluten G12 ELISA Test from Romer Labs, known as the ELISA test, was applied to determine the gluten content in grams per 100 grams of food. Results: Thirty-six family members from the study group and 62 family members from the control group participated, and 72 foods were analyzed. The median total gluten intake for the study group was 9.10 g for the Overbeek method and 13.91 g for the ELISA, significantly lower than the median total gluten intake for the control group of 12.58 g for the Overbeek method and 26.29 g for the ELISA. Moreover, total gluten consumption measured by the Overbeek method was statistically lower than that measured by ELISA. The study and control groups primarily consumed bread and pasta, as determined by ELISA analysis. Conclusions: The daily gluten consumption of family members in the study group was lower than that of family members in the control group despite both groups consuming bread and pasta more frequently. Even though first-degree relatives in the study group reduced their gluten intake, it did not impact on the adherence of their children to the diet. Wheat flour was used significantly less in the homes of the study group compared to the control group, where almost all participants had access to this ingredient. The Overbeek method detected a lower total amount of gluten in the food compared to the ELISA method.
Descrição
Citação
SOUZA, Marilia Graziela Alves de. Consumo de glúten dos genitores de crianças e adolescentes com doença celíaca: avaliação quantitativa por dois métodos. 2024. 87 f. Tese (Doutorado em Pediatria e Ciências Aplicadas à Pediatria) - Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP). São Paulo, 2024.