Avaliação da fragmentação de DNA espermático após processamento seminal por microfluidos (ZyMōt ®) e gradiente descontínuo de densidade e swim up
Data
2024-11-19
Autores
Jordão, Larissa Alves 
Orientadores
Antoniassi, Mariana Pereira
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Objetivo: Avaliar se as técnicas de microfluidos (ZyMōt ®) e gradiente descontínuo de densidade e swim up diminuem os níveis de fragmentação de DNA espermático. Métodos: Foram analisadas amostras de 37 pacientes atendidos na clínica Huntington Eugin - Medicina Reprodutiva, recrutados através de critérios clínicos de inclusão e exclusão. Realizou-se um estudo comparativo da fragmentação de DNA espermático, antes e após a realização das técnicas de ZyMöt ® e de gradiente descontínuo de densidade associado ao swim up. As amostras seminais coletadas foram avaliadas na câmara de Makler, processadas e utilizadas para a técnica de SCSA. A comparação dos dados do espermograma (concentração, motilidade e morfologia) foi realizada pelo Teste t pareado (valores após ZyMõt ® x valores após gradiente de densidade descontínuo + swim up). Para os dados de fragmentação de DNA, a comparação foi realizada pelo teste de Wilcoxon pareado (valores DFI após ZyMõt ® x valores DFI após gradiente de densidade descontínuo + swim up) e pelo Teste t pareado (valores HDS após ZyMõt ® x valores HDS após gradiente de densidade descontínuo + swim up). Os resultados cujos valores p < 0,05 foram considerados estatisticamente significativos. Resultados: Foram observadas diferenças significativas quanto à concentração, à motilidade e à morfologia espermática. Além disso, quando comparados os valores de fragmentação de DNA, foi demonstrado que o ZyMōt ® separou espermatozoides com menores níveis de DFI e HDS. Conclusão: Os resultados obtidos permitiram considerar que o ZyMōt ® pode ser uma técnica eficaz devido à redução dos níveis de fragmentação de DNA espermático. Além disso, os dados encontrados puderam contribuir com novas evidências a nível clínico e laboratorial para as pesquisas acerca da infertilidade, e podem ser aplicados no atendimento e cuidado à saúde masculina.
Objective: To evaluate whether microfluidic techniques (ZyMōt ®) and discontinuous density gradient and swim up reduce sperm DNA fragmentation levels. Methods: Samples from 37 patients treated at the Huntington Eugin Clinic - Reproductive Medicine, recruited through clinical inclusion and exclusion criteria, were tested. A comparative study of sperm DNA fragmentation was performed before and after the ZyMöt ® and discontinuous density gradient techniques associated with swim up. The seminal samples collected were evaluated in the Makler chamber, processed and used for the SCSA technique. Semen analysis (concentration, motility and morphology) was compared using paired t-test (values after ZyMōt ® x values after discontinuous density gradient + swim up). For DNA fragmentation data, the comparison was made using the paired Wilcoxon test (DFI values after ZyMōt ® x DFI values after discontinuous density gradient + swim up) and the paired t-test (HDS values after ZyMōt ® x HDS values after discontinuous density gradient + swim up). Results with p values < 0.05 were considered statistically significant. Results: Significant differences were observed in sperm concentration, motility and morphology. Furthermore, when comparing DNA fragmentation values, it was demonstrated that ZyMōt ® separated sperm with lower levels of DFI and HDS. Conclusion: The results obtained allowed us to consider that ZyMōt ® may be an effective technique due to the reduction in sperm DNA fragmentation levels. Furthermore, the data found could contribute new clinical and laboratory evidence for research on infertility, and can be applied in male health care and care.
Objective: To evaluate whether microfluidic techniques (ZyMōt ®) and discontinuous density gradient and swim up reduce sperm DNA fragmentation levels. Methods: Samples from 37 patients treated at the Huntington Eugin Clinic - Reproductive Medicine, recruited through clinical inclusion and exclusion criteria, were tested. A comparative study of sperm DNA fragmentation was performed before and after the ZyMöt ® and discontinuous density gradient techniques associated with swim up. The seminal samples collected were evaluated in the Makler chamber, processed and used for the SCSA technique. Semen analysis (concentration, motility and morphology) was compared using paired t-test (values after ZyMōt ® x values after discontinuous density gradient + swim up). For DNA fragmentation data, the comparison was made using the paired Wilcoxon test (DFI values after ZyMōt ® x DFI values after discontinuous density gradient + swim up) and the paired t-test (HDS values after ZyMōt ® x HDS values after discontinuous density gradient + swim up). Results with p values < 0.05 were considered statistically significant. Results: Significant differences were observed in sperm concentration, motility and morphology. Furthermore, when comparing DNA fragmentation values, it was demonstrated that ZyMōt ® separated sperm with lower levels of DFI and HDS. Conclusion: The results obtained allowed us to consider that ZyMōt ® may be an effective technique due to the reduction in sperm DNA fragmentation levels. Furthermore, the data found could contribute new clinical and laboratory evidence for research on infertility, and can be applied in male health care and care.
Descrição
Citação
JORDÃO, Larissa Alves. Avaliação da fragmentação de DNA espermático após processamento seminal por microfluidos (ZyMōt ®) e gradiente descontínuo de densidade e swim up. 2024. 78 f. Dissertação (Mestrado em Urologia) - Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP), São Paulo, 2024.