Characterization of cadmium plasma membrane transport in gills of a mangrove crab Ucides cordatus
Data
2014-12-01
Autores
Zanotto, F. P. 
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Artigo
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Resumo
Membrane pathway for intracellular cadmium (Cd2+) accumulation is not fully elucidated in many organisms and has not been studied in crab gill cells. To characterize membrane Cd2+ transport of anterior and posterior gill cells of Ucides cordatus, a hypo-hyper-regulating crab, a change in intracellular Cd2+ concentration under various experimental conditions was examined by using FluoZin, a fluorescent probe. the membrane Cd2+ transport was estimated by the augmentation of FluoZin fluorescence induced by extracellular application of CdCl2 and different inhibitors. Addition of extracellular calcium (Cd2+) to the cells affected little the fluorescence of FluoZin, confirming that Cd2+ was the main ion increasing intracellular fluorescence. Cd2+ channels blockers (nimodipine and verapamil) decreased Cd2+ influx as well as vanadate, a Cd2+-ATPase blocker. Chelating intracellular Cd2+ (BAPTA) decreased Cd2+ influx in gill cells, while increasing intracellular Cd2+ (caffeine) augmented Cd influx. Cd2+ and ATP added at different temporal conditions were not effective at increasing intracellular Cd2+ accumulation. Ouabain (Na+/K+-ATPase inhibitor) increased Cd2+ influx probably through a change in intracellular Na and/or a change in cell membrane potential. Routes of Cd2+ influx, a non-essential metal, through the gill cell plasma membrane of crabs are suggested. (C) 2014 Elsevier B.V. All rights reserved.
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Aquatic Toxicology. Amsterdam: Elsevier B.V., v. 157, p. 21-29, 2014.