Characterization of cadmium plasma membrane transport in gills of a mangrove crab Ucides cordatus

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dc.contributor.authorOrtega, P.
dc.contributor.authorCustodio, M. R.
dc.contributor.authorZanotto, F. P. [UNIFESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:38:15Z
dc.date.available2016-01-24T14:38:15Z
dc.date.issued2014-12-01
dc.description.abstractMembrane pathway for intracellular cadmium (Cd2+) accumulation is not fully elucidated in many organisms and has not been studied in crab gill cells. To characterize membrane Cd2+ transport of anterior and posterior gill cells of Ucides cordatus, a hypo-hyper-regulating crab, a change in intracellular Cd2+ concentration under various experimental conditions was examined by using FluoZin, a fluorescent probe. the membrane Cd2+ transport was estimated by the augmentation of FluoZin fluorescence induced by extracellular application of CdCl2 and different inhibitors. Addition of extracellular calcium (Cd2+) to the cells affected little the fluorescence of FluoZin, confirming that Cd2+ was the main ion increasing intracellular fluorescence. Cd2+ channels blockers (nimodipine and verapamil) decreased Cd2+ influx as well as vanadate, a Cd2+-ATPase blocker. Chelating intracellular Cd2+ (BAPTA) decreased Cd2+ influx in gill cells, while increasing intracellular Cd2+ (caffeine) augmented Cd influx. Cd2+ and ATP added at different temporal conditions were not effective at increasing intracellular Cd2+ accumulation. Ouabain (Na+/K+-ATPase inhibitor) increased Cd2+ influx probably through a change in intracellular Na and/or a change in cell membrane potential. Routes of Cd2+ influx, a non-essential metal, through the gill cell plasma membrane of crabs are suggested. (C) 2014 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv São Paulo, Dept Fisiol, Inst Biociencias, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biofis, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Biofis, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 2009/15546-3
dc.format.extent21-29
dc.identifierhttp://dx.doi.org/10.1016/j.aquatox.2014.09.006
dc.identifier.citationAquatic Toxicology. Amsterdam: Elsevier B.V., v. 157, p. 21-29, 2014.
dc.identifier.doi10.1016/j.aquatox.2014.09.006
dc.identifier.issn0166-445X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38506
dc.identifier.wosWOS:000346547800003
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofAquatic Toxicology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectUcides cordatusen
dc.subjectCadmium transporten
dc.subjectGill cellsen
dc.subjectFluoZinen
dc.subjectCalcium channel blockersen
dc.subjectCell transport inhibitorsen
dc.titleCharacterization of cadmium plasma membrane transport in gills of a mangrove crab Ucides cordatusen
dc.typeinfo:eu-repo/semantics/article
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