Inquérito sorológico para SARS-CoV-2 e detecção viral de SARS-CoV-2 e Herpesvírus em crianças e adolescentes em terapia renal substitutiva e seus acompanhantes conviventes
Data
2024-09-10
Autores
Morais, Richarlisson Borges de 
Orientadores
Taminato, Monica
Tipo
Tese de doutorado
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Objetivo: Verificar a soroprevalência de COVID-19 em uma coorte de crianças e adolescentes em terapia renal substitutiva e comparar com a excreção oral de SARS-CoV-2 e Herpesvírus entre os pacientes e seus acompanhantes conviventes. Métodos: Estudo tipo coorte prospectivo, que analisou amostra de sangue para o teste Anti-SARS-CoV-2 e saliva para a reação em cadeia da polimerase com transcrição reversa (RT-PCR) de SARS-CoV-2 e Herpesvírus. Resultados: Foram incluídos 82 participantes no estudo: 20 (24,4%) são do grupo hemodiálise, sendo 10 pacientes e seus respectivos acompanhantes conviventes, e 62 (75,6%) pertencem ao grupo transplante renal, 31 pacientes e seus acompanhantes. No grupo hemodiálise a soroprevalência de SARS-CoV-2 de 20% para os pacientes. Ao comparar pacientes e acompanhantes, notou-se diferença significativa para Epstein-Barr vírus – EBV (p= 0,0235), Citomegalovírus – CMV (p= 0,0350), Herpesvírus Humano 6 - HHV-6 (p= 0,0253) e Herpesvírus Humano 7 - HHV-7 (p= 0,0317). O número de réplicas virais foi diferente para EBV (p= 0,0012) e HHV-6 (<0,0001), com maior carga viral entre os pacientes. Os transplantados renais apresentaram prevalência para SARS-CoV-2 de 77,4% e ao compara-los a seus acompanhantes, notou-se diferença significativa para sorologia de SARS-CoV-2, EBV, CMV, HHV-6. Conclusões: A soroprevalência de COVID-19 nos grupos de crianças e adolescentes em hemodiálise e transplantados renais, bem como em seus acompanhantes conviventes, foi identificada. Além disso, foi possível identificar a excreção oral de Herpesvírus, encontrando réplicas virais de HSV-1, EBV, CMV, HSV-6 e HSV-7. No grupo de participantes submetidos ao transplante renal evidencia-se relação entre a sorologia de SARS-CoV-2 e o CMV.
Objective: To verify the prevalence of serum COVID-19 in a cohort of children and adolescents on renal replacement therapy and compare it with the oral excretion of SARS-CoV-2 and Herpesvirus among patients and their companions. Methods: Prospective cohort study, which analyzed blood samples for the Anti-SARS-CoV-2 test and saliva for reverse transcription polymerase chain reaction (RT-PCR) of SARS-CoV-2 and Herpesvirus. Results: 82 participants were included in the study: 20 (24.4%) are in the hemodialysis group, 10 patients and their respective companions, and 62 (75.6%) belong to the kidney transplant group, 31 patients and their companions. In the hemodialysis group, serum prevalence of SARS-CoV-2 was 20% for patients. When comparing patients and companions, a significant difference was observed for Epstein-Barr virus - EBV (p= 0.0235), Cytomegalovirus - CMV (p= 0.0350), Human Herpesvirus 6 - HHV-6 (p= 0.0253) and Human Herpesvirus 7 - HHV-7 (p= 0.0317). The number of viral replicates was different for EBV (p= 0.0012) and HHV-6 (<0.0001), with a higher viral load among patients. Kidney transplant recipients had a prevalence of SARS-CoV-2 of 77.4% and when comparing them to their companions, a significant difference was noted for serology of SARS-CoV-2, EBV, CMV, HHV-6. Conclusions: The prevalence of COVID-19 in the groups of children and adolescents on hemodialysis and kidney transplant recipients, as well as in their companions, was identified. In addition, it was possible to identify the oral excretion of Herpesvirus, finding viral replicates of HSV-1, EBV, CMV, HSV-6 and HSV-7. In the group of participants who underwent kidney transplantation, there was a relationship between SARS-CoV-2 serology and CMV.
Objective: To verify the prevalence of serum COVID-19 in a cohort of children and adolescents on renal replacement therapy and compare it with the oral excretion of SARS-CoV-2 and Herpesvirus among patients and their companions. Methods: Prospective cohort study, which analyzed blood samples for the Anti-SARS-CoV-2 test and saliva for reverse transcription polymerase chain reaction (RT-PCR) of SARS-CoV-2 and Herpesvirus. Results: 82 participants were included in the study: 20 (24.4%) are in the hemodialysis group, 10 patients and their respective companions, and 62 (75.6%) belong to the kidney transplant group, 31 patients and their companions. In the hemodialysis group, serum prevalence of SARS-CoV-2 was 20% for patients. When comparing patients and companions, a significant difference was observed for Epstein-Barr virus - EBV (p= 0.0235), Cytomegalovirus - CMV (p= 0.0350), Human Herpesvirus 6 - HHV-6 (p= 0.0253) and Human Herpesvirus 7 - HHV-7 (p= 0.0317). The number of viral replicates was different for EBV (p= 0.0012) and HHV-6 (<0.0001), with a higher viral load among patients. Kidney transplant recipients had a prevalence of SARS-CoV-2 of 77.4% and when comparing them to their companions, a significant difference was noted for serology of SARS-CoV-2, EBV, CMV, HHV-6. Conclusions: The prevalence of COVID-19 in the groups of children and adolescents on hemodialysis and kidney transplant recipients, as well as in their companions, was identified. In addition, it was possible to identify the oral excretion of Herpesvirus, finding viral replicates of HSV-1, EBV, CMV, HSV-6 and HSV-7. In the group of participants who underwent kidney transplantation, there was a relationship between SARS-CoV-2 serology and CMV.
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MORAIS, Richarlisson Borges de. Inquérito sorológico para SARS-CoV-2 e detecção viral de SARS-CoV-2 e Herpesvírus em crianças e adolescentes em terapia renal substitutiva e seus acompanhantes conviventes. 2024. 168 f. Tese (Doutorado em Enfermagem) - Escola Paulista de Enfermagem, Universidade Federal de São Paulo (UNIFESP), São Paulo, 2024.