Impacto das próteses sintéticas na atividade in vitro da metaloproteinase-2 nas linhagens de fibroblastos de indivíduos com hérnia inguinal indireta
Arquivos
Data
2009
Autores
Marcondes, Wagner 
Orientadores
Goldenberg, Alberto
Tipo
Tese de doutorado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Objetivo: Avaliar o impacto das próteses de polipropileno (PPL) e polipropileno/
poliglecaprone (PPL/PG) na atividade in vitro da metaloproteinase-2 (MMP-2) em
fibroblastos de indivíduos com hérnia inguinal indireta. Métodos: A partir de amostras
de pele e saco herniário de pacientes com hérnia inguinal indireta unilateral formam-se
linhagens de fibroblastos encubadas em três meios distintos: fibroblastos isolados,
fibroblastos com tela de PPL e fibroblastos com tela de PPL/PG. A atividade da MMP-2
foi avaliada pela zimografia. Resultados: A atividade da MMP-2 nos fibroblastos da
pele é menor quando associada á prótese de PPL (p=<0,0001) e PPL/PG (p=<0,0001).
A atividade da MMP-2 de fibroblastos da pele é menor quando associada com PPL do
que com o PPL/PG (p=<0,0001). A atividade da MMP-2 de fibroblastos da pele é maior
do que a do saco herniário independente da presença (p=<0,0001) ou ausência das
próteses (p=<0,0001). A atividade da MMP-2 nos fibroblastos do saco herniário é maior
quando se associa com as próteses de PPL (p=<0,0001) e PPL/PG (p=<0,0001). A
atividade da MMP-2 de fibroblastos originários do saco herniário é maior quando se
associa com a prótese de PPL/PG do que com a prótese de PPL (p=0,0328).
Conclusões: As próteses de PPL e PPL/PG diminuem a atividade da MMP-2 nos
fibroblastos de pele e aumentam a atividade da MMP-2 nos fibroblastos de saco
herniário nas linhagens celulares formadas a partir de tecidos de indivíduos com hérnia
inguinal indireta unilateral.
Aim: This study aims to evaluate in fibroblasts obtained from individuals with indirect hernia the influence of different surgical meshes [polypropylene (PPL) and polypropylene / polyglecaprone (PPL/PG)] in the in vitro activity of the metalloproteinase-2 (MMP-2). Methods: Fibroblasts lineages were created from samples of the skin and the hernial sac. The cells were cultured in 3 different environments: (a) fibroblasts only, (b) fibroblast + PPL mesh, and (c) fibroblasts + PPL/PG mesh. MMP-2 activity was measured by zymography. Results: MMP-2 activity is decreased in skin fibroblasts cultured with PPL (p=<.0001) and PPL/PG (p=<.0001) meshes when compared to isolated fibroblasts. MMP-2 activity is lower in PPL mesh group compared to the PPL/PG group (p=<.0001). MMP-2 activity is higher in skin fibroblasts compared to hernial sac lineages irrespective of the presence (p=<.0001) or absence (p=<.0001) of the meshes. MMP-2 activity is decreased in hernial sac fibroblasts cultured with PPL (p=<.0001) and PPL/PG (p=<.0001) meshes when compared to isolated fibroblasts. MMP-2 activity is higher in PPL mesh group compared to the PPL/PG group (p=<.0328). Conclusions: MMP-2 activity is decreased in skin fibroblasts cultured with PPL and PPL/PG meshes but increased in hernial sac fibroblasts cultured with PPL and PPL/PG meshes.
Aim: This study aims to evaluate in fibroblasts obtained from individuals with indirect hernia the influence of different surgical meshes [polypropylene (PPL) and polypropylene / polyglecaprone (PPL/PG)] in the in vitro activity of the metalloproteinase-2 (MMP-2). Methods: Fibroblasts lineages were created from samples of the skin and the hernial sac. The cells were cultured in 3 different environments: (a) fibroblasts only, (b) fibroblast + PPL mesh, and (c) fibroblasts + PPL/PG mesh. MMP-2 activity was measured by zymography. Results: MMP-2 activity is decreased in skin fibroblasts cultured with PPL (p=<.0001) and PPL/PG (p=<.0001) meshes when compared to isolated fibroblasts. MMP-2 activity is lower in PPL mesh group compared to the PPL/PG group (p=<.0001). MMP-2 activity is higher in skin fibroblasts compared to hernial sac lineages irrespective of the presence (p=<.0001) or absence (p=<.0001) of the meshes. MMP-2 activity is decreased in hernial sac fibroblasts cultured with PPL (p=<.0001) and PPL/PG (p=<.0001) meshes when compared to isolated fibroblasts. MMP-2 activity is higher in PPL mesh group compared to the PPL/PG group (p=<.0328). Conclusions: MMP-2 activity is decreased in skin fibroblasts cultured with PPL and PPL/PG meshes but increased in hernial sac fibroblasts cultured with PPL and PPL/PG meshes.
Descrição
Citação
MARCONDES, Wagner. Impacto das próteses sintéticas na atividade in vitro da metaloproteinase-2 nas linhagens de fibroblastos de indivíduos com hérnia inguinal indireta. 2009. 71 f. Tese (Doutorado em Ciências) - Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, 2009.